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Molecular recognition of B-DNA by Hoechst 33258

Harshman, Keith D. and Dervan, Peter B. (1985) Molecular recognition of B-DNA by Hoechst 33258. Nucleic Acids Research, 13 (13). pp. 4825-4835. ISSN 0305-1048.

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The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl EDTA·Fe(II) [MPE·Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five ± one bp in size and rich in A·T DNA base pairs. The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. Hydrophobic interaction of the flanking phenol and N-methylpiperazine rings would afford a steric blockade of one additional base pair on each side.

Item Type:Article
Additional Information:Copyright © 1985 Oxford University Press. Received 18 March 1985; Revised and Accepted 27 May 1985. Supported by the American Cancer Society research grant Number NP-428.
Record Number:CaltechAUTHORS:HARnar85
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2271
Deposited By: Tony Diaz
Deposited On:21 Mar 2006
Last Modified:26 Dec 2012 08:48

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