Harshman, Keith D. and Dervan, Peter B. (1985) Molecular recognition of B-DNA by Hoechst 33258. Nucleic Acids Research, 13 (13). pp. 4825-4835. ISSN 0305-1048. PMCID PMC321829. http://resolver.caltech.edu/CaltechAUTHORS:HARnar85
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The binding sites of Hoechst 33258, netropsin and distamycin on three DNA restriction fragments from plasmid pBR322 were compared by footprinting with methidiumpropyl EDTA·Fe(II) [MPE·Fe(II)]. Hoechst, netropsin and distamycin share common binding sites that are five ± one bp in size and rich in A·T DNA base pairs. The five base pair protection patterns for Hoechst may result from a central three base pair recognition site bound by two bisbenzimidazole NHs forming a bridge on the floor of the minor groove between adjacent adenine N3 and thymine O2 atoms on opposite helix strands. Hydrophobic interaction of the flanking phenol and N-methylpiperazine rings would afford a steric blockade of one additional base pair on each side.
|Additional Information:||© 1985 Oxford University Press. Received 18 March 1985; Revised and Accepted 27 May 1985. Supported by the American Cancer Society research grant Number NP-428.|
|PubMed Central ID:||PMC321829|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||21 Mar 2006|
|Last Modified:||09 May 2016 18:23|
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