Andrews, Nancy C. and Baltimore, David (1986) Lack of evidence for VPg priming of poliovirus RNA synthesis in the host factor-dependent in vitro replicase reaction. Journal of Virology, 58 (1). pp. 212-215. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:ANDjvir86
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Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detected with only the host factor present.
|Additional Information:||Copyright © 1986 by the American Society for Microbiology. Received 30 September 1985/Accepted 25 November 1985 We thank M. Baron for anti-VPg antiserum. N.C.A. was partially supported by an M.S.T.P. training grant from the National Institute for General Medical Sciences (2T 32 GM07753-06); this work was also supported by Public Health Service grant A122346 from the National Institute of Allergy and Infectious Diseases to D.B.|
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|Deposited On:||21 Mar 2006|
|Last Modified:||26 Dec 2012 08:48|
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