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The minimum transforming region of v-abl is the segment encoding protein-tyrosine kinase

Prywes, Ron and Foulkes, J. Gordon and Baltimore, David (1985) The minimum transforming region of v-abl is the segment encoding protein-tyrosine kinase. Journal of Virology, 54 (1). pp. 114-122. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:PRYjvir85a

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Abstract

Only 1.2 kilobases (kb) at the 5' end of the 3.9-kb v-abl sequence in Abelson murine leukemia virus is required for fibroblast transformation. A precise delineation of this minimum transforming region was made by using small 5' or 3' deletions. Insertions of four amino acids, generated by putting synthetic DNA linkers into various restriction enzyme cleavage sites, abolished transforming activity, indicating that much of the internal sequence of the minimum transforming region plays a critical role in the transformation process. This 5' 1.2 kb of v-abl encodes protein-tyrosine kinase activity when expressed in Escherichia coli. Each of the mutations which caused a loss of transformation activity also resulted in a loss of protein-tyrosine kinase activity when expressed in E. coli. The minimum transforming region of v-abl contains amino acid homology to other protein-tyrosine kinase oncogenes, and a comparison with these oncogenes is presented.


Item Type:Article
Additional Information:Copyright © 1985 by the American Society for Microbiology. Received 22 October 1984/Accepted 28 December 1984 This work was supported by Public Health Service grant CA26717 from the National Cancer Institute.
Record Number:CaltechAUTHORS:PRYjvir85a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:PRYjvir85a
Alternative URL:http://jvi.asm.org/cgi/content/abstract/54/1/114
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2352
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:29 Mar 2006
Last Modified:26 Dec 2012 08:48

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