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Purification and Thermal Stability of Intact Bacillus subtilis Flagella

Dimmitt, K. and Simon, M. (1971) Purification and Thermal Stability of Intact Bacillus subtilis Flagella. Journal of Bacteriology, 105 (1). pp. 369-375. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:DIMjbact71a

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Abstract

Flagella were prepared and purified in a relatively intact form from bacterial lysates. Immunochemical tests showed that over 95% of the protein in the final preparation consisted of flagellar antigen. These flagella are more stable to thermal denaturation than flagella filaments obtained by shearing. Their thermal properties more closely resemble those of flagella in the native state on bacteria. The presence of the hook structure is responsible for this extra stability.


Item Type:Article
Additional Information:Copyright © 1971 American Society for Microbiology. Received for publication 8 June 1970 This research was supported by the National Science Foundation research grant GB-15655.
Record Number:CaltechAUTHORS:DIMjbact71a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DIMjbact71a
Alternative URL:http://jb.asm.org/cgi/content/abstract/105/1/369
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2373
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:31 Mar 2006
Last Modified:26 Dec 2012 08:48

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