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Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks

Dimmitt, K. and Simon, M. I. (1971) Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks. Journal of Bacteriology, 108 (1). pp. 282-286. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:DIMjbact71b

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Abstract

A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.


Item Type:Article
Additional Information:Copyright © 1971 American Society for Microbiology. Received for publication 7 July 1971 This investigation was supported by grant no. GB-15655 from the National Science Foundation. Presented in part at the Xth International Congress of Microbiology, Mexico City, August 1970.
Record Number:CaltechAUTHORS:DIMjbact71b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DIMjbact71b
Alternative URL:http://jb.asm.org/cgi/content/abstract/108/1/282
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2374
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:31 Mar 2006
Last Modified:26 Dec 2012 08:48

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