Dimmitt, K. and Simon, M. I. (1971) Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks. Journal of Bacteriology, 108 (1). pp. 282-286. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:DIMjbact71b
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A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.
|Additional Information:||Copyright © 1971 American Society for Microbiology. Received for publication 7 July 1971 This investigation was supported by grant no. GB-15655 from the National Science Foundation. Presented in part at the Xth International Congress of Microbiology, Mexico City, August 1970.|
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|Deposited On:||31 Mar 2006|
|Last Modified:||26 Dec 2012 08:48|
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