Swulius, Matthew T. and Chen, Songye and Ding, H. Jane and Li, Zhuo and Briegel, Ariane and Pilhofer, Martin and Tocheva, Elitza I. and Lybarger, Suzanne R. and Johnson, Tanya L. and Sandkvist, Maria and Jensen, Grant J. (2011) Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria. Biochemical and Biophysical Research Communications, 407 (4). pp. 650-655. ISSN 0006-291X http://resolver.caltech.edu/CaltechAUTHORS:20110524-100904457
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How rod-shaped bacteria form and maintain their shape is an important question in bacterial cell biology. Results from fluorescent light microscopy have led many to believe that the actin homolog MreB and a number of other proteins form long helical filaments along the inner membrane of the cell. Here we show using electron cryotomography of six different rod-shaped bacterial species, at macromolecular resolution, that no long (>80 nm) helical filaments exist near or along either surface of the inner membrane. We also use correlated cryo-fluorescent light microscopy (cryo-fLM) and electron cryo-tomography (ECT) to identify cytoplasmic bundles of MreB, showing that MreB filaments are detectable by ECT. In light of these results, the structure and function of MreB must be reconsidered: instead of acting as a large, rigid scaffold that localizes cell-wall synthetic machinery, moving MreB complexes may apply tension to growing peptidoglycan strands to ensure their orderly, linear insertion.
|Additional Information:||© 2011 Elsevier Inc. Received 4 March 2011; Available online 16 March 2011. We thank Drs. Jian Shi and Alasdair McDowall for support of the EM instrumentation. This work was supported by NIH grant R01 GM094800B to G.J.J. and the Gordon and Betty Moore Center for Integrative Study of Cell Regulation. Work done by M.S. was supported by NIH grant RO1 AI049294.|
|Subject Keywords:||MreB; Electron cryo-tomography; Helical filament; Rod-shaped; Bacterial shape|
|Official Citation:||Matthew T. Swulius, Songye Chen, H. Jane Ding, Zhuo Li, Ariane Briegel, Martin Pilhofer, Elitza I. Tocheva, Suzanne R. Lybarger, Tanya L. Johnson, Maria Sandkvist, Grant J. Jensen, Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria, Biochemical and Biophysical Research Communications, Volume 407, Issue 4, 22 April 2011, Pages 650-655, ISSN 0006-291X, DOI: 10.1016/j.bbrc.2011.03.062.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||24 May 2011 20:05|
|Last Modified:||24 May 2011 20:05|
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