Shih, Chi-Yuan and Chen, Yang and Li, Wei and Xie, Jun and He, Qing and Tai, Yu-Chong (2006) An integrated system for on-chip temperature gradient interaction chromatography. Sensors and Actuators A, 127 (2). pp. 207-215. ISSN 0924-4247 http://resolver.caltech.edu/CaltechAUTHORS:20110602-101105257
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This paper reports the first integrated MEMS high performance liquid chromatography (HPLC) chip that consists of a parylene high-pressure liquid chromatography (LC) column, an electrochemical sensor, a resistive heater and a thermal-isolation structure for on-chip temperature gradient interaction chromatography (TGIC) application. Two sets of devices for different chromatography criteria were fabricated. The separation column was 8-mm long, 100-μm wide, 25-μm high and was packed with 5-μm sized, C18-coated beads using conventional slurry-packing technique. A novel parylene-enhanced air-gap thermal isolation technology was used to reduce heater power consumption by 58% and to reduce temperature rise in the off-column area by 67%. To test the chromatography performance of the fabricated system, a mixture of derivatized amino acids was chosen for separation. A temporal temperature gradient scanning from 25 °C to 65 °C with a ramping rate of 3.6 °C/min was applied to the column during separation. Successful chromatographic separation of derivatized amino acids was obtained on the chip. Compared with conventional temperature gradient HPLC system which incorporates “macro oven” to generate temporal temperature gradient on the column, our chip's thermal performance, i.e., power consumption and thermal response, is greatly improved without sacrificing chromatography quality.
|Additional Information:||© 2005 Elsevier B.V. Received 23 February 2005; revised 20 July 2005; accepted 26 August 2005. Available online 29 September 2005. This work was supported by the NSF Center for Neuromorphic Systems Engineering (CNSE) at Caltech. The authors would also like to thank Mr. Trevor Roper for his assistance in processing.|
|Subject Keywords:||temperature gradient; lab-on-a-chip; parylene; amino acid; thermal isolation; electrochemical sensing|
|Official Citation:||Chi-Yuan Shih, Yang Chen, Wei Li, Jun Xie, Qing He, Yu-Chong Tai, An integrated system for on-chip temperature gradient interaction chromatography, Sensors and Actuators A: Physical, Volume 127, Issue 2, MEMS 2005 Special Issue - Special Issue of the Micromechanics Section of Sensors and Actuators (SAMM), based on contributions revised from the technical digest of the IEEE 18th International Conference on Micro Electro Mechanical Systems (MEMS-2005)., 13 March 2006, Pages 207-215, ISSN 0924-4247, DOI: 10.1016/j.sna.2005.08.013. (http://www.sciencedirect.com/science/article/pii/S0924424705004656)|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||02 Jun 2011 18:34|
|Last Modified:||02 Jun 2011 18:34|
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