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Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures

Putzer, K. P. and Buchholz, L. A. and Lidstrom, M. E. and Remsen, C. C. (1991) Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures. Applied and Environmental Microbiology, 57 (12). pp. 3656-3659. ISSN 0099-2240. http://resolver.caltech.edu/CaltechAUTHORS:PUTaem91

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Abstract

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.


Item Type:Article
Additional Information:Copyright © 1991, American Society for Microbiology. Received 19 April 1991/Accepted 5 October 1991 We acknowledge those who contributed to this work in one way or another: Steve Konings, a partner in the Menomonee River work; Marilyn Schaller for assistance with TEM; and Barb Wimpee for some of the illustrations. This work was supported in part by the Center for Great Lakes Studies and in part by grant 2022-1RP-79 from the NASA Interdisciplinary Research Program in Earth Sciences. Contribution no. 355 from the Center for Great Lakes Studies.
Record Number:CaltechAUTHORS:PUTaem91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:PUTaem91
Alternative URL:http://aem.asm.org/cgi/content/abstract/57/12/3656
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2394
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:03 Apr 2006
Last Modified:26 Dec 2012 08:48

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