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Computational design and biochemical characterization of maize nonspecific lipid transfer protein variants for biosensor applications

Choi, Eun Jung and Mao, Jessica and Mayo, Stephen L. (2007) Computational design and biochemical characterization of maize nonspecific lipid transfer protein variants for biosensor applications. Protein Science, 16 (4). pp. 582-588. ISSN 0961-8368. http://resolver.caltech.edu/CaltechAUTHORS:20110614-141918849

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Abstract

Lipid transfer proteins (LTPs) are a family of proteins that bind and transfer lipids. Utilizing the maize LTP, we have successfully engineered fluorescent reagentless biosensors for the natural ligand of LTPs; this was achieved by using computational protein design to remove a disulfide bridge and attaching a thio-reactive fluorophore. Conformational change induced by ligand titration is thought to affect the fluorescence of the fluorophore, allowing detection of ligand binding. Fluorescence measurements show that our LTP variants have affinity to palmitate that is consistent with wild-type LTP. These molecules have the potential to be utilized as scaffolds to design hydrophobic ligand biosensors or to serve as drug carriers.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1110/ps.062607007 DOIUNSPECIFIED
http://onlinelibrary.wiley.com/doi/10.1110/ps.062607007/abstractPublisherUNSPECIFIED
Contact Email Address:steve@mayo.caltech.edu
Additional Information:© 2007 The Protein Society. Published by Cold Spring Harbor Laboratory Press. Received October 9, 2006; Final Revision October 9, 2006; Accepted December 9, 2006. Article first published online: 2 Jan. 2009. We thank Marie Ary for editing the manuscript. This work was supported by the Howard Hughes Medical Institute, the Ralph M. Parsons Foundation, the Army Research Office (Institute of Collaborative Biotechnologies), and an IBM Shared University Research Grant.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Army Research Office (ARO) Institute for Collaborative Biotechnologies UNSPECIFIED
IBM Shared University UNSPECIFIED
Subject Keywords:Biosensing Techniques, 2-Naphthylamine, Circular Dichroism, Carrier Proteins, Models: Molecular, Zea mays, Spectrometry: Fluorescence
Record Number:CaltechAUTHORS:20110614-141918849
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110614-141918849
Official Citation:Choi, E. J., Mao, J. and Mayo, S. L. (2007), Computational design and biochemical characterization of maize nonspecific lipid transfer protein variants for biosensor applications. Protein Science, 16: 582–588. doi: 10.1110/ps.062607007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24006
Collection:CaltechAUTHORS
Deposited By: Benjamin Perez
Deposited On:21 Jun 2011 22:43
Last Modified:23 Aug 2016 10:02

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