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A designed apoplastocyanin variant that shows reversible folding

Datta, Deepshikha and Mayo, Stephen L. (2002) A designed apoplastocyanin variant that shows reversible folding. Biochemical and Biophysical Research Communications, 296 (4). pp. 988-990. ISSN 0006-291X. http://resolver.caltech.edu/CaltechAUTHORS:20110620-160423337

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Abstract

Plastocyanin, like many other metalloproteins, does not undergo reversible folding, which is thought to be due to an irreversible conformational change in the copper-binding site. Moreover, apoplastocyanin's ability to adopt a native tertiary structure is highly salt-dependent, and even in high salt, it has an irreversible thermal denaturation. Here, we report a designed apoplastocyanin variant, PCV, that is well folded and has reversible folding in both high and low salt conditions. This variant provides a tractable model for understanding and designing protein β-sheets.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0006-291X(02)02037-5DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0006291X02020375PublisherUNSPECIFIED
Additional Information:© 2002 Elsevier Science. Received 31 July 2002; Available online 21 August 2002.
Subject Keywords:Temperature; Copper; Circular Dichroism; Escherichia coli; Apoproteins; Plant Proteins; Crystallography: X-Ray; Plastocyanin; Salts; Protein Folding; Mutagenesis: Site-Directed; Protein Conformation; Protein Structure: Secondary; Binding Sites; Protein Structure: Tertiary; Plastocyanin; Reversible folding; Copper-binding site; Protein design
Record Number:CaltechAUTHORS:20110620-160423337
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110620-160423337
Official Citation:Deepshikha Datta, Stephen L Mayo, A designed apoplastocyanin variant that shows reversible folding, Biochemical and Biophysical Research Communications, Volume 296, Issue 4, 30 August 2002, Pages 988-990, ISSN 0006-291X, 10.1016/S0006-291X(02)02037-5.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24077
Collection:CaltechAUTHORS
Deposited By: Marie Ary
Deposited On:03 Oct 2011 22:46
Last Modified:03 Oct 2011 22:46

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