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Immunoglobulin gene rearrangements in normal mouse B cells

Early, P. and Nottenburg, C. and Weissman, I. and Hood, L. (1982) Immunoglobulin gene rearrangements in normal mouse B cells. Molecular and Cellular Biology, 2 (7). pp. 829-836. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:EARmcb82

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Abstract

We have analyzed the structure of rearranged mu heavy-chain genes obtained from the genomic DNA of normal BALB/c mouse spleen cells expressing surface immunoglobulin M. Examples were found of two types of nonproductive rearrangements, which may be responsible for allelic exclusion in normal B cells. In one of these rearrangements, a germ line D gene segment has joined to the JH4 gene segment but no V/D joining has occurred. We present evidence that D gene segments lie as a cluster between V and J gene segments in the germ line. A comparison of conserved sequences in V and D gene segments suggests that the D gene segments, which are found only in the heavy-chain gene family, may have evolved from V gene segments similar to the Vk family.


Item Type:Article
Additional Information:Copyright © 1982 by the American Society for Microbiology. Received 19 November 1981/Accepted 19 March 1982 This work was supported by Public Health Service grants AI 16913 (to L.H.) GM 07276 (to I.W.), and AI 09072 (to I. W.) from the National Institutes of Health and by American Cancer Society grant IM 56 to I.W. P.E. was supported by a National Institutes of Health postdoctoral fellowship. We thank Bob Coffman for antisera and Francis Asissi for FACS assistance.
Record Number:CaltechAUTHORS:EARmcb82
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:EARmcb82
Alternative URL:http://mcb.asm.org/cgi/content/abstract/2/7/829
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ID Code:2408
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Deposited On:03 Apr 2006
Last Modified:26 Dec 2012 08:48

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