CaltechAUTHORS
  A Caltech Library Service

Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants

Morgan, Chantal S. and Holton, James M. and Olafson, Barry D. and Bjorkman, Pamela J. and Mayo, Stephen L. (1997) Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants. Protein Science, 6 (8). pp. 1771-1773. ISSN 0961-8368 http://resolver.caltech.edu/CaltechAUTHORS:20110620-160424679

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20110620-160424679

Abstract

Class I major histocompatibility complex (MHC) molecules bind peptides derived from degraded proteins for display to T cells of the immune system. Peptides bind to MHC proteins with varying affinities, depending upon their sequence and length. We demonstrate that the thermal stability of the MHC-peptide complex depends directly on peptide binding affinity. We use this correlation to develop a convenient method to determine peptide dissociation constants by measuring MHC-peptide complex stability using thermal denaturation profiles monitored by circular dichroism.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/pro.5560060819DOIUNSPECIFIED
http://onlinelibrary.wiley.com/doi/10.1002/pro.5560060819/abstractPublisherUNSPECIFIED
Additional Information:© 1997 The Protein Society. Received December 26, 1996; Accepted April 23, 1997. Article first published online: 31 Dec. 2008. We thank B. Dahiyat, G. Hathaway, J. Johnson, and D. Penny for technical help, C. White for insightful discussions, and M. Ary for comments on the manuscript. C.S.M. is supported by the James Irvine Foundation and ARCS Foundation. P.J.B. acknowledges support from the Arthritis Foundation. S.L.M. acknowledges support from the Rita Allen Foundation, the David and Lucile Packard Foundation, and the Searle Scholars Program/The Chicago Community Trust.
Subject Keywords:Animals; Hot Temperature; H-2 Antigens; Amino Acid Sequence; Recombinant Proteins; Peptides; Circular Dichroism; Protein Denaturation; Cricetinae; Protein Binding; CHO Cells; classI MHC; dissociation constant
Record Number:CaltechAUTHORS:20110620-160424679
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110620-160424679
Official Citation:Morgan, C. S., Holton, J. M., Olafson, B. D., Bjorkman, P. J. and Mayo, S. L. (1997), Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants. Protein Science, 6: 1771–1773. doi: 10.1002/pro.5560060819
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24081
Collection:CaltechAUTHORS
Deposited By: Marie Ary
Deposited On:30 Sep 2011 16:59
Last Modified:30 Sep 2011 16:59

Repository Staff Only: item control page