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A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins

Datta, Deepshikha and Wang, Pin and Carrico, Isaac S. and Mayo, Stephen L. and Tirrell, David A. (2002) A designed phenylalanyl-tRNA synthetase variant allows efficient in vivo incorporation of aryl ketone functionality into proteins. Journal of the American Chemical Society, 124 (20). pp. 5652-5653. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20110620-160431532

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Abstract

Incorporation of non-natural amino acids into proteins in vivo expands the scope of protein synthesis and design. p-Acetylphenylalanine was incorporated into recombinant dihydrofolate reductase (DHFR) in Escherichia coli via a computationally designed mutant form of the phenylalanyl-tRNA synthetase of the host. DHFR outfitted with ketone functionality can be chemoselectively ligated with hydrazide reagents under mild conditions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0177096 DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0177096PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Contact Email Address:tirrell@caltech.edu
Additional Information:© 2002 American Chemical Society. Received December 7, 2001. Publication Date (Web): April 26, 2002. The authors thank William A. Goddard III, Nagarajan Vaidehi, Kent Kirshenbaum, and Yi Tang for helpful discussions. This work was supported by NIH Grants R01-GM62523 and T32-GM08501, the NSF Center for the Science and Engineering of Materials at Caltech, the Howard Hughes Medical Institute, the Ralph M. Parsons Foundation, and an IBM shared University Research Grant.
Funders:
Funding AgencyGrant Number
NIHR01-GM62523
NIHT32-GM08501
NSF Center for the Science and Engineering of Materials at the California Institute of TechnologyUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
IBM Shared University Research GrantUNSPECIFIED
Subject Keywords:Escherichia coli; Phenylalanine; Ketones; Recombinant Proteins; Phenylalanine-tRNA Ligase; Mutagenesis
Record Number:CaltechAUTHORS:20110620-160431532
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110620-160431532
Official Citation:A Designed Phenylalanyl-tRNA Synthetase Variant Allows Efficient in Vivo Incorporation of Aryl Ketone Functionality into Proteins Deepshikha Datta,Pin Wang,Isaac S. Carrico,Stephen L. Mayo, and and David A. Tirrell Journal of the American Chemical Society 2002 124 (20), 5652-5653
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24112
Collection:CaltechAUTHORS
Deposited By: Marie Ary
Deposited On:27 Sep 2011 21:13
Last Modified:16 Jan 2015 19:47

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