Bolon, Daniel N. and Marcus, Joshua S. and Ross, Scott A. and Mayo, Stephen L. (2003) Prudent modeling of core polar residues in computational protein design. Journal of Molecular Biology, 329 (3). pp. 611-622. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20110620-160434663
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20110620-160434663
Hydrogen bond interactions were surveyed in a set of protein structures. Compared to surface positions, polar side-chains at core positions form a greater number of intra-molecular hydrogen bonds. Furthermore, the majority of polar side-chains at core positions form at least one hydrogen bond to main-chain atoms that are not involved in hydrogen bonds to other main-chain atoms. Based on this structural survey, hydrogen bond rules were generated for each polar amino acid for use in protein core design. In the context of protein core design, these prudent polar rules were used to eliminate from consideration polar amino acid rotamers that do not form a minimum number of hydrogen bonds. As an initial test, the core of Escherichia coli thioredoxin was selected as a design target. For this target, the prudent polar strategy resulted in a minor increase in computational complexity compared to a strategy that did not allow polar residues. Dead-end elimination was used to identify global minimum energy conformations for the prudent polar and no polar strategies. The prudent polar strategy identified a protein sequence that was thermodynamically stabilized by 2.5 kcal/mol relative to wild-type thioredoxin and 2.2 kcal/mol relative to a thioredoxin variant whose core was designed without polar residues.
|Contact Email Address:||firstname.lastname@example.org|
|Additional Information:||© 2003 Elsevier Science Ltd. Received 26 February 2003; accepted 19 March 2003. Edited by M. Levitt. Available online 21 May 2003. We thank P. S. Shah for aid in protein expression and purification, P. Strop for helpful discussions and M. Ary for critical comments on the manuscript. This research was supported by the Howard Hughes Medical Institute and the Ralph M. Parsons Foundation (S.L.M.), the Helen G. and Arthur McCallum Foundation, the Evelyn Sharp Graduate Fellowship, and grant GM07616 from the National Institutes of Health (D.N.B.).|
|Subject Keywords:||Protein Denaturation, Escherichia coli, Thermodynamics, Models: Molecular, Hydrogen Bonding, Nuclear Magnetic Resonance: Biomolecular, Thioredoxins, Computer Simulation, Static Electricity, Hydrophobic and Hydrophilic Interactions, Amino Acid Sequence, Molecular Sequence Data, Circular Dichroism|
|Non-Subject Keywords:||protein design; hydrogen bond; energy function; thermodynamic stability; protein core|
|Official Citation:||Daniel N. Bolon, Joshua S. Marcus, Scott A. Ross, Stephen L. Mayo, Prudent Modeling of Core Polar Residues in Computational Protein Design, Journal of Molecular Biology, Volume 329, Issue 3, 6 June 2003, Pages 611-622, ISSN 0022-2836, DOI: 10.1016/S0022-2836(03)00423-6. (http://www.sciencedirect.com/science/article/pii/S0022283603004236)|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Marie Ary|
|Deposited On:||22 Jun 2011 18:11|
|Last Modified:||23 Aug 2016 10:02|
Repository Staff Only: item control page