Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library
Abstract
The utility of blue fluorescent protein (BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield (0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.
Additional Information
© 2006 Nature Publishing Group. Received 5 July; accepted 11 October; published online 19 November 2006. The authors thank K. Dane, A. Nguyen, P. Bessette and C. Gottstein for helpful discussions and Alexander Mikhailovsky for assistance with fluorescence lifetime measurements, and acknowledge support from National Institutes of Health–National Institute of Biomedical Imaging and Bioengineering grant EB-000205. Author Contributions: M.A.M., T.P.T., S.L.M. and P.S.D. designed research, M.A.M. and T.P.T. performed research, T.P.T. and S.L.M. contributed computational tools, M.A.M., T.P.T. and P.S.D. analyzed data, and M.A.M. and P.S.D. wrote the paper.Attached Files
Supplemental Material - Fig1.pdf
Supplemental Material - Fig2.pdf
Supplemental Material - Fig3.pdf
Supplemental Material - Fig4.pdf
Supplemental Material - Fig5.pdf
Supplemental Material - Fig6.pdf
Supplemental Material - Fig7.pdf
Supplemental Material - Fig8.pdf
Supplemental Material - Methods.pdf
Supplemental Material - Table1.pdf
Supplemental Material - Table2.pdf
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Additional details
- Eprint ID
- 24132
- Resolver ID
- CaltechAUTHORS:20110620-160435158
- NIH
- EB-000205
- Created
-
2011-06-21Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field