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A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function

Budd, Martin E. and Campbell, Judith L. (1997) A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Molecular and Cellular Biology, 17 (4). pp. 2136-2142. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:BUDmcb97

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Abstract

We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication. We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase. FEN-1 is implicated in DNA replication and repair in yeast, and the mammalian homolog of yFEN-1 (DNase IV, FEN-1, or MF1) participates in Okazaki fragment maturation. Overproduction of yFEN-1, encoded by RAD27/RTH1, suppresses the temperature-sensitive growth of dna2-1 mutants. Overproduction of Dna2 suppresses the rad27/rth1 delta temperature-sensitive growth defect. dna2-1 rad27/rth1 delta double mutants are inviable, indicating that the mutations are synthetically lethal. The genetic interactions are likely due to direct physical interaction between the two proteins, since both epitope-tagged yFEN-1 and endogenous yFEN-1 coimmunopurify with tagged Dna2. The simplest interpretation of these data is that one of the roles of Dna2 helicase is associated with processing of Okazaki fragments.


Item Type:Article
Additional Information:Copyright © 1997, American Society for Microbiology Received 25 October 1996/Returned for modification 3 December 1996/Accepted 7 January 1997 This work was supported by NIH grant GM25508 and NSF grant 9507352.
Record Number:CaltechAUTHORS:BUDmcb97
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BUDmcb97
Alternative URL:http://mcb.asm.org/cgi/content/abstract/17/4/2136
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ID Code:2414
Collection:CaltechAUTHORS
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Deposited On:03 Apr 2006
Last Modified:26 Dec 2012 08:49

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