Budd, Martin E. and Campbell, Judith L. (1995) DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae. Molecular and Cellular Biology, 15 (4). pp. 2173-2179. ISSN 0270-7306 http://resolver.caltech.edu/CaltechAUTHORS:BUDmcb95
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The ability of yeast DNA polymerase mutant strains to carry out repair synthesis after UV irradiation was studied by analysis of postirradiation molecular weight changes in cellular DNA. Neither DNA polymerase alpha, delta, epsilon, nor Rev3 single mutants evidenced a defect in repair. A mutant defective in all four of these DNA polymerases, however, showed accumulation of single-strand breaks, indicating defective repair. Pairwise combination of polymerase mutations revealed a repair defect only in DNA polymerase delta and epsilon double mutants. The extent of repair in the double mutant was no greater than that in the quadruple mutant, suggesting that DNA polymerases alpha and Rev3p play very minor, if any, roles. Taken together, the data suggest that DNA polymerases delta and epsilon are both potentially able to perform repair synthesis and that in the absence of one, the other can efficiently substitute. Thus, two of the DNA polymerases involved in DNA replication are also involved in DNA repair, adding to the accumulating evidence that the two processes are coupled.
|Additional Information:||Copyright © 1995, American Society for Microbiology Received 12 October 1994/Returned for modification 7 December 1994/Accepted 26 January 1995 This work was supported by grants from the USPHS (GM25508 and GM47281) and the state of California (TRDRP 3RT-0190).|
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