Aizenberg, Mark and Schuman, Erin M. (2011) Cerebellar-Dependent Learning in Larval Zebrafish. Journal of Neuroscience, 31 (24). pp. 8708-8712. ISSN 0270-6474 http://resolver.caltech.edu/CaltechAUTHORS:20110705-132153102
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Understanding how neuronal network activity contributes to memory formation is challenged by the complexity of most brain circuits and the restricted ability to monitor the activity of neuronal populations in vivo. The developing zebrafish (Danio rerio) is an animal model that circumvents these problems, because zebrafish larvae possess a rich behavioral repertoire and an accessible brain. Here, we developed a classical conditioning paradigm in which 6- to 8-d-old larvae develop an enhanced motor response to a visual stimulus (conditioned stimulus, CS) when it is paired with touch (unconditioned stimulus, US). Using in vivo calcium imaging we demonstrate that CS and US activate different subsets of neurons in the cerebellum; their activity, modulated by learning two-photon laser ablation, revealed that the cerebellum is involved in acquisition and extinction, but not the retention, of this memory.
|Additional Information:||© 2011 the authors. Received Dec. 16, 2010; revised March 25, 2011; accepted April 15, 2011. Funding from the Gordon and Betty Moore Foundation supported this work. We gratefully acknowledge Ueli Rutishauser, Flora Hinz, and Gilles Laurent for comments on the manuscript. We thank Sally Kim for help with the imaging set-up and all Schuman group members for helpful discussions and criticism. Author contributions: M.A. and E.M.S. designed research; M.A. performed research; M.A. analyzed data; M.A. and E.M.S. wrote the paper.|
|Official Citation:||Mark Aizenberg and Erin M. Schuman Cerebellar-Dependent Learning in Larval Zebrafish The Journal of Neuroscience, 15 June 2011, 31(24):8708-8712; doi:10.1523/JNEUROSCI.6565-10.2011|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||05 Jul 2011 20:41|
|Last Modified:||26 Dec 2012 13:22|
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