Shi, Tie-Jun Sten and Hua, Xiao-ying and Lu, Xiaoying and Malkmus, Shelle and Kinney, Jeff and Holmberg, Kristina and Wirz, Sebastian and Ceccatelli, Sandra and Yaksh, Tony and Bartfai, Tamas and Hökfelt, Tomas (2006) Sensory neuronal phenotype in galanin receptor 2 knockout mice: focus on dorsal root ganglion neurone development and pain behaviour. European Journal of Neuroscience, 23 (3). pp. 627-636. ISSN 0953-816X http://resolver.caltech.edu/CaltechAUTHORS:20110719-153530731
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Galanin is a 29-amino-acid peptide expressed in dorsal root ganglion (DRG) neurones and spinal dorsal horn neurones. It affects pain threshold and has developmental and trophic effects. Galanin acts at three G-protein-coupled receptors, galanin receptors (GalR1–3), each expressed in the DRGs as suggested by in situ hybridization and/or reverse transcriptase-polymerase chain reaction. The GalR2 knockout (–/–) mice permit studies on the contributions of this receptor subtype to the role of galanin at the spinal level. At 1 week after sciatic nerve transection (axotomy), there were 16–20% fewer neurones in intact and contralateral DRGs of –/– mice as compared with wild-type (WT) mice. In addition, a significant neurone loss (26% reduction) was found in the ipsilateral DRGs of WT mice, whereas no further neurone loss was seen in –/– mice. Expression of several peptides has been examined after axotomy, including galanin, neuropeptide Y and two of its receptors as well as substance P, and no significant differences were found between –/– and WT mice in either ipsi- or contralateral DRGs, respectively. After thermal injury and spinal nerve ligation, onset and duration of hyperalgesia in the injured paw were similar in GalR2–/– and WT animals. Recovery from spinal nerve ligation-caused allodynia had the same kinetics in –/– and WT animals. These data are in line with earlier observations from the peripheral and central nervous system, suggesting that galanin actions mediated by GalR2 subtype are of importance in neurodevelopment and neuroprotection.
|Additional Information:||© 2006 The Authors. Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd. Received 26 August 2005, revised 22 November 2005, accepted 27 November 2005. Article first published online: 16 Feb. 2006. This study was supported by the Marianne and Marcus Wallenberg Foundation, Knut and Alice Wallenberg Foundation, Swedish Research Council (04X-2887 and 33X-10815), The Swedish Research Council for Environment, Agricultural Sciences and Special Planning, The Swedish Brain Foundation (T.-J.S.S), NIH NS 41954 (to T.Y.) and NIMH grant R01MH63080-04 (to T.B.).|
|Subject Keywords:||CGRP; nerve injury; neurone loss; neuropeptides; NPY; substance P|
|Official Citation:||Shi, T.-J. S., Hua, X.-y., Lu, X., Malkmus, S., Kinney, J., Holmberg, K., Wirz, S., Ceccatelli, S., Yaksh, T., Bartfai, T. and Hökfelt, T. (2006), Sensory neuronal phenotype in galanin receptor 2 knockout mice: focus on dorsal root ganglion neurone development and pain behaviour. European Journal of Neuroscience, 23: 627–636. doi: 10.1111/j.1460-9568.2006.04593.x|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||20 Jul 2011 14:08|
|Last Modified:||20 Jul 2011 14:08|
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