Ackerson, Christopher J. and Jadzinsky, Pablo D. and Jensen, Grant J. and Kornberg, Roger D. (2006) Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates. Journal of the American Chemical Society, 128 (8). pp. 2635-2640. ISSN 0002-7863 http://resolver.caltech.edu/CaltechAUTHORS:20110803-092706578
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A general method of rigid, specific labeling of proteins with gold clusters has been devised. The method relies on the conjugation of a glutathione monolayer-protected gold cluster (MPC) with a single chain Fv antibody fragment (scFv), mutated to present an exposed cysteine residue. Efficient formation of a gold-thiolate bond between the MPC and scFv depends on activation of the gold cluster by chemical oxidation. Once formed, the MPC-scFv conjugate is treated with a reductant to quench cluster reactivity. The procedure has been performed with an MPC with an average Au_(71) core and an scFv directed against a tetrameric protein, the influenza neuraminidase. A complex of the MPC-scFv conjugate with the neuraminidase was isolated, and the presence of four gold clusters was verified by cryoelectron microscopy.
|Additional Information:||© 2006 American Chemical Society. Received August 28, 2005. Publication Date (Web): February 2, 2006. We thank Christopher Chidsey for many helpful discussions. pGC-NC10 was a kind gift from Olan Dolezal. C.J.A. received support from NIH training grant T32 GM0829. This research was supported by NIH grants AI21144 and NIH GM63025.|
|Official Citation:||Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates Christopher J. Ackerson,, Pablo D. Jadzinsky,, Grant J. Jensen, and, Roger D. Kornberg, Journal of the American Chemical Society 2006 128 (8), 2635-2640|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||George Porter|
|Deposited On:||03 Aug 2011 16:49|
|Last Modified:||26 Dec 2012 13:26|
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