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Electron Cryotomography of the E. coli Pyruvate and 2-Oxoglutarate Dehydrogenase Complexes

Murphy, Gavin E. and Jensen, Grant J. (2005) Electron Cryotomography of the E. coli Pyruvate and 2-Oxoglutarate Dehydrogenase Complexes. Structure, 13 (12). pp. 1765-1773. ISSN 0969-2126. http://resolver.caltech.edu/CaltechAUTHORS:20110803-110300203

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Abstract

The E. coli pyruvate and 2-oxoglutarate dehydrogenases are two closely related, large complexes that exemplify a growing number of multiprotein “machines” whose domains have been studied extensively and modeled in atomic detail, but whose quaternary structures have remained unclear for lack of an effective imaging technology. Here, electron cryotomography was used to show that the E1 and E3 subunits of these complexes are flexibly tethered ~11 nm away from the E2 core. This result demonstrates unambiguously that electron cryotomography can reveal the relative positions of features as small as 80 kDa in individual complexes, elucidating quaternary structure and conformational flexibility.


Item Type:Article
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http://dx.doi.org/10.1016/j.str.2005.08.016DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S096921260500359XPublisherUNSPECIFIED
Additional Information:Copyright © 2005 Elsevier. Received 27 May 2005; revised 27 July 2005; accepted 1 August 2005. Published: December 13, 2005. Available online 13 December 2005. We thank T. Wagenknecht and J. Berkowitz for the protein samples and A. Rawlinson for help preparing the figures. This work was supported in part by National Institutes of Health Grant PO1 GM66521 to G.J.J., Department of Energy grant DE-FG02-04ER63785 to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Ralph M. Parsons Foundation, the Agouron Institute, and the Gordon and Betty Moore Foundation. Accession Numbers: One extracted PDHC particle and one OGDHC particle were submitted to the EM Data Bank with the accession codes EMD-1151 and EMD-1152, respectively.
Funders:
Funding AgencyGrant Number
NIHPO1 GM66521
Department of Energy (DOE)DE-FG02-04ER63785
Caltech Beckman InstituteUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20110803-110300203
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110803-110300203
Official Citation:Gavin E. Murphy, Grant J. Jensen, Electron Cryotomography of the E. coli Pyruvate and 2-Oxoglutarate Dehydrogenase Complexes, Structure, Volume 13, Issue 12, December 2005, Pages 1765-1773, ISSN 0969-2126, DOI: 10.1016/j.str.2005.08.016. (http://www.sciencedirect.com/science/article/pii/S096921260500359X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24653
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Aug 2011 18:46
Last Modified:03 Aug 2011 18:46

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