CaltechAUTHORS
  A Caltech Library Service

A Micro Device for Separation of Erythrocytes and Leukocytes in Human Blood

Zheng, Siyang and Tai, Yu-Chong and Kasdan, Harvey (2005) A Micro Device for Separation of Erythrocytes and Leukocytes in Human Blood. In: 2005 27th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Proceedings of Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE , Piscataway, NJ, pp. 1024-1027. ISBN 0-7803-8740-6 http://resolver.caltech.edu/CaltechAUTHORS:20110829-095853628

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20110829-095853628

Abstract

We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 µm lateral separation was achieved for 5 µm and 10 µm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1109/IEMBS.2005.1616592 DOIUNSPECIFIED
http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=1616592PublisherUNSPECIFIED
Additional Information:© 2005 IEEE. Issue Date: 17-18 Jan. 2006. Date of Current Version: 10 April 2006. This work is supported by NASA through National Space Biomedical Research Institute (NSBRI). The cooperative agreement number is NCC 9-58-317.
Funders:
Funding AgencyGrant Number
National Space Biomedical Research Institute (NSBRI)NCC 9-58-317
Other Numbering System:
Other Numbering System NameOther Numbering System ID
INSPEC Accession Number9218705
Record Number:CaltechAUTHORS:20110829-095853628
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110829-095853628
Official Citation:Siyang Zheng; Yu-Chong Tai; Kasdan, H.; , "A Micro Device for Separation of Erythrocytes and Leukocytes in Human Blood," Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the , vol., no., pp.1024-1027, 17-18 Jan. 2006 doi: 10.1109/IEMBS.2005.1616592 URL: http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1616592&isnumber=33900
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25141
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:31 Aug 2011 21:14
Last Modified:31 Aug 2011 21:14

Repository Staff Only: item control page