Bond, Vincent C. and Wold, Barbara (1993) Nucleolar localization of myc transcripts. Molecular and Cellular Biology, 13 (6). pp. 3221-3230. ISSN 0270-7306 http://resolver.caltech.edu/CaltechAUTHORS:BONmcb93
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In situ hybridization has revealed a striking subnuclear distribution of c-myc RNA transcripts. A major fraction of the sense-strand nuclear c-myc transcripts was localized to the nucleoli. myc intron 1-containing RNAs were noticeably absent from nucleoli, accumulating instead in the nucleoplasm. The localization of myc RNA to nucleoli was shown to be common to a number of diverse cell types, including primary Sertoli cells and several cell lines. Furthermore, nucleolar localization was not restricted to c-myc and N-myc and myoD transcripts also displayed this phenomenon. In contrast, gamma-actin or lactate dehydrogenase transcripts did not display nucleolar localization. These observations suggest a new role for the nucleolus in transport and/or turnover of potential mRNAs.
|Additional Information:||Copyright © 1993 by the American Society for Microbiology. Received 16 December 1992/Returned for modification 10 January 1993/Accepted 19 February 1993 We thank Jesus del Mazo and Ellen Rothenberg for their assistance with in situ hybridization techniques. We also thank Jeff Miner, Paul Garrity, and Sean Tavtigian of the Wold group for hybridization probes. Finally, we thank John Abelson, Richard Axel, and members of the Wold group for their comments on the manuscript. This work was supported by grants from the Lucille Markey Charitable Trust and the National Institutes of Health.|
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|Deposited On:||06 Apr 2006|
|Last Modified:||26 Dec 2012 08:49|
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