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NMR and temperature-jump measurements of de novo designed proteins demonstrate rapid folding in the absence of explicit selection for kinetics

Gillespie, Blake and Vu, Dung M. and Shah, Premal S. and Marshall, Shannon A. and Dyer, R. Brian and Mayo, Stephen L. and Plaxco, Kevin W. (2003) NMR and temperature-jump measurements of de novo designed proteins demonstrate rapid folding in the absence of explicit selection for kinetics. Journal of Molecular Biology, 330 (4). pp. 813-819. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20110913-173426521

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Abstract

We address the importance of natural selection in the origin and maintenance of rapid protein folding by experimentally characterizing the folding kinetics of two de novo designed proteins, NC3-NCAP and ENH-FSM1. These 51 residue proteins, which adopt the helix-turn-helix homeodomain fold, share as few as 12 residues in common with their most closely related natural analog. Despite the replacement of up to 3/4 of their residues by a computer algorithm optimizing only thermodynamic properties, the designed proteins fold as fast or faster than the 35,000 s^(-1) observed for the closest natural analog. Thus these de novo designed proteins, which were produced in the complete absence of selective pressures or design constraints explicitly aimed at ensuring rapid folding, are among the most rapidly folding proteins reported to date.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(03)00616-8 DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0022283603006168PublisherUNSPECIFIED
Additional Information:© 2003 Elsevier Ltd. Received 23 January 2003; revised 8 May 2003; Accepted 8 May 2003. Edited by C. R. Matthews. Available online 1 July 2003. The authors gratefully acknowledge the assistance of Jerry Hu, Scott Ross, Dan Raleigh, and Rick Dahlquist regarding various aspects of the NMR measurements reported here. This work was supported by NIH grant R01GM62868-01A1 (KWP), BioSTAR grant s97-79 (KWP), NIH grant GM053640 (RBD) and ACS junior postdoctoral research fellowship ACS CD INC 2-5-00 (BG).
Funders:
Funding AgencyGrant Number
NIHR01GM62868-01A1
BioSTARs97-79
NIHGM053640
ACS junior postdoctoral research fellowshipACS CD INC 2-5-00
Subject Keywords:Kinetics; Protein Binding; Protein Folding; Algorithms; Molecular Sequence Data; Protein Structure: Tertiary; Urea; Magnetic Resonance Spectroscopy; Dose-Response Relationship: Drug; Temperature; Escherichia coli; Time Factors; Amino Acid Sequence; Thermodynamics; Sequence Homology: Amino Acid; Software; evolution; two-state; NMR lineshape
Record Number:CaltechAUTHORS:20110913-173426521
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110913-173426521
Official Citation:Blake Gillespie, Dung M. Vu, Premal S. Shah, Shannon A. Marshall, R.Brian Dyer, Stephen L. Mayo, Kevin W. Plaxco, NMR and Temperature-jump Measurements of de Novo Designed Proteins Demonstrate Rapid Folding in the Absence of Explicit Selection for Kinetics, Journal of Molecular Biology, Volume 330, Issue 4, 18 July 2003, Pages 813-819, ISSN 0022-2836, 10.1016/S0022-2836(03)00616-8.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25335
Collection:CaltechAUTHORS
Deposited By: Marie Ary
Deposited On:23 Sep 2011 22:26
Last Modified:23 Sep 2011 22:26

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