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A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration

Coles, Edward and Christiansen, Jeff and Economou, Androulla and Wilkinson, David G. and Bronner, Marianne E. (2004) A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration. Development, 131 (21). pp. 5309-5317. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20110920-103005137

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Abstract

Previous work has revealed that proteins that bind to bone morphogenetic proteins (BMPs) and inhibit their signalling have a crucial role in the spatial and temporal regulation of cell differentiation and cell migration by BMPs. We have identified a chick homologue of crossveinless 2, a Drosophila gene that was identified in genetic studies as a promoter of BMP-like signalling. Chick Cv-2 has a conserved structure of five cysteine-rich repeats similar to those found in several BMP antagonists, and a C-terminal Von Willebrand type D domain. Cv-2 is expressed in the chick embryo in a number of tissues at sites at which elevated BMP signalling is required. One such site of expression is premigratory neural crest, in which at trunk levels threshold levels of BMP activity are required to initiate cell migration. We show that, when overexpressed, Cv-2 can weakly antagonise BMP4 activity in Xenopus embryos, but that in other in vitro assays Cv-2 can increase the activity of co-expressed BMP4. Furthermore, we find that increased expression of Cv-2 causes premature onset of trunk neural crest cell migration in the chick embryo, indicative of Cv-2 acting to promote BMP activity at an endogenous site of expression. We therefore propose that BMP signalling is modulated both by antagonists and by Cv-2 that acts to elevate BMP activity.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.01419 DOIUNSPECIFIED
http://dev.biologists.org/content/131/21/5309.abstractPublisherUNSPECIFIED
Additional Information:© 2004 The Company of Biologists. Published online before print September 29, 2004. We thank Dalit Sela-Donenfeld and Siew-Lan Ang for comments on the manuscript, Yi-Chuan Cheng for chick Sox10 probe, Mike Jones for Xbra probe, Elizabeth Robertson for BMP4-Myc expression construct, Ian Harragan for histological sectioning, Lisa Taneyhill for advice on immunoprecipitations, and Nobue Itasaki and Branko Latinkic for advice on Xenopus microinjections.
Subject Keywords:BMP activity, Neural crest, Cell migration, Chick, Xenopus
Record Number:CaltechAUTHORS:20110920-103005137
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110920-103005137
Official Citation:Edward Coles, Jeff Christiansen, Androulla Economou, Marianne Bronner-Fraser, and David G. Wilkinson A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration Development 2004 131:5309-5317; published ahead of print September 29, 2004, doi:10.1242/dev.01419
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25364
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 Sep 2011 20:11
Last Modified:26 Dec 2012 13:41

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