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Effect of Pactamycin on Synthesis of Poliovirus Proteins: a Method for Genetic Mapping

Taber, Robert and Rekosh, David and Baltimore, David (1971) Effect of Pactamycin on Synthesis of Poliovirus Proteins: a Method for Genetic Mapping. Journal of Virology, 8 (4). pp. 395-401. ISSN 0022-538X.

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We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5' -> 3') of NCVP 1, NCVP X, NCVP 2.

Item Type:Article
Additional Information:Copyright © 1971 American Society for Microbiology. Received for publication 24 June 1971 We thank Harvey Lodish for his helpful criticisms and discussions and for making large amounts of (35S] methionine available to us. This investigation was supported by Public Health Service grant AI-08388 from the National Institute of Alergy and Infectious Diseases. R. T. was a postdoctoral fellow and D. B. was a Faculty Research Awardee of the American Cancer Society.
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ID Code:2673
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Deposited On:15 Apr 2006
Last Modified:26 Dec 2012 08:50

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