Fu, Anne Y. and Spence, Charles and Scherer, Axel and Arnold, Frances H. and Quake, Stephen R. (1999) A microfabricated fluorescence-activated cell sorter. Nature Biotechnology, 17 (11). pp. 1109-1111. ISSN 1087-0156. http://resolver.caltech.edu/CaltechAUTHORS:20111101-071340837
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We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (µFACS) for sorting various biological entities. Compared with conventional FACS machines, the µFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used µFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
|Additional Information:||© 1999 Nature America Inc. Received 12 May 1999; accepted 19 August 1999. This work was supported in part by the Army Research Office and the National Science Foundation. We especially thank Hou-Pu Chou for fabrication of the silicon molds.|
|Subject Keywords:||microchips, cell sorting, disposable chip, flow cytometry|
|Official Citation:||A microfabricated fluorescence-activated cell sorter - pp1109 - 1111 Anne Y. Fu, Charles Spence, Axel Scherer, Frances H. Arnold & Stephen R. Quake doi:10.1038/15095|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||01 Nov 2011 14:41|
|Last Modified:||01 Nov 2011 14:41|
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