Fu, Anne Y. and Spence, Charles and Scherer, Axel and Arnold, Frances H. and Quake, Stephen R. (1999) A microfabricated fluorescence-activated cell sorter. Nature Biotechnology, 17 (11). pp. 1109-1111. ISSN 1087-0156 http://resolver.caltech.edu/CaltechAUTHORS:20111101-071340837
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Abstract
We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (µFACS) for sorting various biological entities. Compared with conventional FACS machines, the µFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used µFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.
| Item Type: | Article | ||||||
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| Additional Information: | © 1999 Nature America Inc. Received 12 May 1999; accepted 19 August 1999. This work was supported in part by the Army Research Office and the National Science Foundation. We especially thank Hou-Pu Chou for fabrication of the silicon molds. | ||||||
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| Subject Keywords: | microchips, cell sorting, disposable chip, flow cytometry | ||||||
| Record Number: | CaltechAUTHORS:20111101-071340837 | ||||||
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:20111101-071340837 | ||||||
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| Official Citation: | A microfabricated fluorescence-activated cell sorter - pp1109 - 1111 Anne Y. Fu, Charles Spence, Axel Scherer, Frances H. Arnold & Stephen R. Quake doi:10.1038/15095 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 27536 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Ruth Sustaita | ||||||
| Deposited On: | 01 Nov 2011 14:41 | ||||||
| Last Modified: | 01 Nov 2011 14:41 |
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