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A microfabricated fluorescence-activated cell sorter

Fu, Anne Y. and Spence, Charles and Scherer, Axel and Arnold, Frances H. and Quake, Stephen R. (1999) A microfabricated fluorescence-activated cell sorter. Nature Biotechnology, 17 (11). pp. 1109-1111. ISSN 1087-0156.

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We have demonstrated a disposable microfabricated fluorescence-activated cell sorter (µFACS) for sorting various biological entities. Compared with conventional FACS machines, the µFACS provides higher sensitivity, no cross-contamination, and lower cost. We have used µFACS chips to obtain substantial enrichment of micron-sized fluorescent bead populations of differing colors. Furthermore, we have separated Escherichia coli cells expressing green fluorescent protein from a background of nonfluorescent E. coli cells and shown that the bacteria are viable after extraction from the sorting device. These sorters can function as stand-alone devices or as components of an integrated microanalytical chip.

Item Type:Article
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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1999 Nature America Inc. Received 12 May 1999; accepted 19 August 1999. This work was supported in part by the Army Research Office and the National Science Foundation. We especially thank Hou-Pu Chou for fabrication of the silicon molds.
Funding AgencyGrant Number
Army Research Office (ARO)UNSPECIFIED
Subject Keywords:microchips, cell sorting, disposable chip, flow cytometry
Record Number:CaltechAUTHORS:20111101-071340837
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Official Citation:A microfabricated fluorescence-activated cell sorter - pp1109 - 1111 Anne Y. Fu, Charles Spence, Axel Scherer, Frances H. Arnold & Stephen R. Quake doi:10.1038/15095
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27536
Deposited By: Ruth Sustaita
Deposited On:01 Nov 2011 14:41
Last Modified:13 May 2015 21:51

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