Birdwell, Charles R. and Strauss, James H. (1974) Distribution of the Receptor Sites for Sindbis Virus on the Surface of Chicken and BHK Cells. Journal of Virology, 14 (3). pp. 672-678. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:BIRjvir74a
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Sindbis virus was adsorbed to chicken cells or to BHK cells, and the distribution of virus over the surface of the cell was examined by electron microscopy of surface replicas. The distribution of virus particles on the cell was used to indicate the position of virus receptors at the cell surface. When purified Sindbis virus was adsorbed at 37 C to cells prefixed with glutaraldehyde, the virus particles were evenly distributed over the surface of most cells. There was a large variability from cell to cell, however, in the number of virus particles adsorbed, and regions with different concentrations of virus particles were sometimes observed on the same cell. The concentration of virus receptors observed varied from 20 to 160/µm2 of cell surface, and, thus, the total number of virus receptors per chicken cell is on the order of 105. When virus was adsorbed to unfixed cells at 4 C, the virus particles were clustered into aggregates varying from a few particles to large crystalline arrays (the latter seen only in chicken cells). These conditions are apparently conducive to virus aggregation, and this, coupled with free lateral diffusion of the virus-receptor complex in the cell membrane at 4 C, leads to the observed clustering.
|Additional Information:||Copyright © 1974 American Society for Microbiology. Received for publication 15 April 1974 We are grateful to Jean-Paul Revel for his advice throughout the conduct of these experiments, and to Ellen Strauss for her aid in preparing the manuscript. Edith Lenches and Sharman Christoph provided excellent technical assistance. This work was supported by Public Health Service grant GM 06965 from the National Institute of General Medical Sciences and by grant GB 31763X from the National Science Foundation. C. R. B. was supported by Public Health Service Training Grant GM00086 from the National Institute of General Medical Sciences.|
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|Deposited On:||26 Apr 2006|
|Last Modified:||26 Dec 2012 08:50|
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