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Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB

Ohtsubo, Eiichi and Hsu, Ming-Ta (1978) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. Journal of Bacteriology, 134 (3). pp. 778-794. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:OHTjbact78a

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Abstract

The genetic and physical structures of commonly used F-prime factors carrying the galactose region of the Escherichia coli chromosome were analyzed. Deletions in the chromosomal DNA sequences in the F-prime factors were found to be frequent events. A genetic method was developed to reconstruct the original F-prime factors from deletion variants. Heteroduplex analysis of the reconstructed F-prime factors confirmed the derivation of the F-prime factors F100 and F152, from the same Hfr, and finally determined the normal E. coli chromosomal sequence in the region between fep and uvrB, containing about 5 min in genetic units and about 246.5 in kilobase units (kb). This sequence could be connected with the DNA sequences of the lac-purE region, which had been physically determined previously. Together they constituted a total of 528.6 kb. From these combined sequences, the distance from lacPO to galK was calculated to be 412.9 kb, which corresponds to 8.8 min in genetic units.


Item Type:Article
Additional Information:Copyright © 1978 by the American Society for Microbiology. Received for publication 7 October 1977 We wish to express our gratitude to Norman Davidson for his generosity in providing his laboratory space and funds (Public Health Service grant GM 10991 from the National Institute of General Medical Sciences). We would also like to thank him and M. Guyer for reading the manuscript in preparation. We thank P. Broda, A. Campbell, W. Epstein, D. Freifelder, T. Fukasawa, J. R. Guest, B. Low, M. Nomura, E. Signer, and I. Young for providing the bacteria strains used in this work.
Record Number:CaltechAUTHORS:OHTjbact78a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OHTjbact78a
Alternative URL:http://jb.asm.org/cgi/content/abstract/134/3/778
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2779
Collection:CaltechAUTHORS
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Deposited On:27 Apr 2006
Last Modified:26 Dec 2012 08:50

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