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Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: isolation of a new F-prime factor, F80, and its implication for the mechanism of F integration into the chromosome

Ohtsubo, Eiichi and Hsu, Ming-Ta (1978) Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: isolation of a new F-prime factor, F80, and its implication for the mechanism of F integration into the chromosome. Journal of Bacteriology, 134 (3). pp. 795-800. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:OHTjbact78b

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Abstract

A new F-prime factor, F80, was isolated from an Escherichia coli strain harboring the F-prime factor F8 by selecting for transfer of the supE marker to a RecA- recipient. Genetic analysis shows that F80 carries a segment of the chromosomal DNA between lip and suc in addition to the tol-gal region normally in F8. Physical analysis by the electron microscope heteroduplex method suggests that the formation of F80 from F8 involves recombination between the alphabeta segment of F, which is present in F8, and the homologous sequence of F present in the E. coli chromosome at the site where F is supposed to integrate to form HfrP3. The implications of this result for the general mechanisms of F integration to form Hfr's are discussed.


Item Type:Article
Additional Information:Copyright © 1978 by the American Society for Microbiology. Received for publication 7 October 1977 We wish to express our gratitude to Norman Davidson for his generosity in providing his laboratory space and funds (Public Health Service grant GM 10991 from the National Institute of General Medical Sciences). We would also like to thank him and M. Guyer for reading the manuscript in preparation.
Record Number:CaltechAUTHORS:OHTjbact78b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OHTjbact78b
Alternative URL:http://jb.asm.org/cgi/content/abstract/134/3/795
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2780
Collection:CaltechAUTHORS
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Deposited On:27 Apr 2006
Last Modified:26 Dec 2012 08:50

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