Mui, Timothy P. and Fuss, Jill O. and Ishida, Justin P. and Tainer, John A. and Barton, Jacqueline K. (2011) ATP-Stimulated, DNA-Mediated Redox Signaling by XPD, a DNA Repair and Transcription Helicase. Journal of the American Chemical Society, 133 (41). pp. 16378-16381. ISSN 0002-7863 http://resolver.caltech.edu/CaltechAUTHORS:20111116-102627735
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Using DNA-modified electrodes, we show DNA-mediated signaling by XPD, a helicase that contains a [4Fe-4S] cluster and is critical for nucleotide excision repair and transcription. The DNA-mediated redox signal resembles that of base excision repair proteins, with a DNA-bound redox potential of ~80 mV versus NHE. Significantly, this signal increases with ATP hydrolysis. Moreover, the redox signal is substrate-dependent, reports on the DNA conformational changes associated with enzymatic function, and may reflect a general biological role for DNA charge transport.
|Additional Information:||© 2011 American Chemical Society. Received: August 1, 2011. Publication Date (Web): September 22, 2011. This research was supported by the NIH (GM49216 to J.K.B. and CA112093 to J.A.T.) and the DOE (ENIGMA program under Contract No. DE-AC02-05CH11231 to J.A.T.). T.P.M. also thanks the NSF for a graduate fellowship.|
|Official Citation:||ATP-Stimulated, DNA-Mediated Redox Signaling by XPD, a DNA Repair and Transcription Helicase Timothy P. Mui, Jill O. Fuss, Justin P. Ishida, John A. Tainer, and Jacqueline K. Barton Journal of the American Chemical Society 2011 133 (41), 16378-16381|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||16 Nov 2011 18:48|
|Last Modified:||26 Dec 2012 14:25|
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