Novella, Isabel and Reissig, Daniel D. and Wilke, Claus O. (2004) Density-Dependent Selection in Vesicular Stomatitis Virus. Journal of Virology, 78 (11). pp. 5799-5804. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:NOVjvir04
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We used vesicular stomatitis virus to test the effect of complementation on the relative fitness of a deleterious mutant, monoclonal antibody-resistant mutant (MARM) N, in competition with its wild-type ancestor. We carried out competitions of MARM N and wild-type populations at different multiplicities of infection (MOIs) and initial ratios of the wild type to the mutant and found that the fitness of MARM N relative to that of the wild type is very sensitive to changes in the MOI (i.e., the degree of complementation) but depends little, if at all, on the initial frequencies of MARM N and the wild type. Further, we developed a mathematical model under the assumption that during coinfection both viruses contribute to a common pool of protein products in the infected cell and that they both exploit this common pool equally. Under such conditions, the fitness of all virions that coinfect a cell is the average fitness in the absence of coinfection of that group of virions. In the absence of coinfection, complementation cannot take place and the relative fitness of each competitor is only determined by the selective value of its own products. We found good agreement between our experimental results and the model predictions, which suggests that the wild type and MARM N freely share all of their gene products under coinfection.
|Additional Information:||Copyright © 2004, American Society for Microbiology. Received 29 October 2003/ Accepted 14 January 2004 Bonnie Ebendick provided excellent technical assistance. Work at the Medical College of Ohio was funded by NIAID (NIH) grant R01-AI45686 to I.S.N., and C.O.W. received support from NSF contract DEB-9981397. Correction. JOURNAL OF VIROLOGY, Apr. 2006, p. 4207. doi:10.1128/JVI.80.8.4207.2006|
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|Deposited On:||27 Apr 2006|
|Last Modified:||26 Dec 2012 08:50|
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