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Process of Infection with Bacteriophage {varphi}X174 XXIV. New Type of Temperature-sensitive Mutant

Dalgarno, L. and Sinsheimer, Robert L. (1968) Process of Infection with Bacteriophage {varphi}X174 XXIV. New Type of Temperature-sensitive Mutant. Journal of Virology, 2 (8). pp. 822-829. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:DALjvir68

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Abstract

A group of temperature-sensitive mutants of {varphi}X174 has been isolated which can go through a single, normal one-step growth cycle at 40 C but fail to form plaques at this temperature. Such mutants fail to initiate a second cycle at 40 C; however they can gain the capacity to infect at 40 C, upon incubation for 10 min in broth at 30 C. In regaining the ability to infect, the phage appear to undergo a temperature-dependent conformational alteration. The inverse process, a reversible loss of ability to infect at 40 C, is observed when such phage produced at 30 C are incubated for 2 hr at 40 C. The defect in initiation of a second cycle of infection appears to be in the injection of viral deoxyribonucleic acid. A two-step complementation test has been used to identify the cistron coding for the affected function. Such mutants are also unusually sensitive to an irreversible thermal inactivation when incubated at 40 C.


Item Type:Article
Additional Information:Copyright © 1968 American Society for Microbiology. Received for publication 7 May 1968 We thank C. A. Hutchison for useful discussions. This investigation was supported in part by Public Health Service grant GM 13554 from the National Institute of General Medical Sciences.
Record Number:CaltechAUTHORS:DALjvir68
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:DALjvir68
Alternative URL:http://jvi.asm.org/cgi/content/abstract/2/8/822
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2799
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:27 Apr 2006
Last Modified:26 Dec 2012 08:51

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