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The Drosophila SH2-SH3 adapter protein Dock is expressed in embryonic axons and facilitates synapse formation by the RP3 motoneuron

Desai, Chand J. and Garrity, Paul A. and Keshishian, Haig and Zipursky, S. Lawrence and Zinn, Kai (1999) The Drosophila SH2-SH3 adapter protein Dock is expressed in embryonic axons and facilitates synapse formation by the RP3 motoneuron. Development, 126 (7). pp. 1527-1535. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20111207-131758750

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Abstract

The Dock SH2-SH3 domain adapter protein, a homolog of the mammalian Nck oncoprotein, is required for axon guidance and target recognition by photoreceptor axons in Drosophila larvae. Here we show that Dock is widely expressed in neurons and at muscle attachment sites in the embryo, and that this expression pattern has both maternal and zygotic components. In motoneurons, Dock is concentrated in growth cones. Loss of zygotic dock function causes a selective delay in synapse formation by the RP3 motoneuron at the cleft between muscles 7 and 6. These muscles often completely lack innervation in late stage 16 dock mutant embryos. RP3 does form a synapse later in development, however, because muscles 7 and 6 are normally innervated in third-instar mutant larvae. The absence of zygotically expressed Dock also results in subtle defects in a longitudinal axon pathway in the embryonic central nervous system. Concomitant loss of both maternally and zygotically derived Dock dramatically enhances these central nervous system defects, but does not increase the delay in RP3 synaptogenesis. These results indicate that Dock facilitates synapse formation by the RP3 motoneuron and is also required for guidance of some interneuronal axons The involvement of Dock in the conversion of the RP3 growth cone into a presynaptic terminal may reflect a role for Dock-mediated signaling in remodeling of the growth cone's cytoskeleton.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/126/7/1527.abstractPublisherUNSPECIFIED
Additional Information:© 1999 The Company of Biologists Limited. Accepted 13 January; published on WWW 3 March 1999. We thank Jim Clemens, Jack Dixon and Mark Perin for antibodies, the Bloomington Stock Center and Corey Goodman’s group for fly strains, and Steven Hanks and Richard Kim for helpful advice. This work was supported by NIH R01 grant NS28182 to K. Z., by startup funds from Vanderbilt University Medical School to C. J. D. and by NIH R01 grant NS31651 to H. K.; P. A. G. was supported by postdoctoral fellowships from the Bank of America-Giannini Foundation and the Leukemia Society of America; S. L. Z. is an Investigator of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIHR01 NS28182
Vanderbilt University Medical SchoolUNSPECIFIED
NIHR01 NS31651
Bank of America-Giannini FoundationUNSPECIFIED
Leukemia Society of AmericaUNSPECIFIED
Howard Hughes Medical InstituteUNSPECIFIED
Subject Keywords:adapter protein; Dock; Nck; neuromuscular junction; growth cone; synaptogenesis; tyrosine phosphorylation; Drosophila
Record Number:CaltechAUTHORS:20111207-131758750
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20111207-131758750
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28353
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Dec 2011 23:36
Last Modified:26 Dec 2012 14:35

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