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Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT

Yip, M. L. Richard and Lamka, Michele L. and Lipshitz, Howard D. (1997) Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT. Development, 124 (11). pp. 2129-2141. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120118-145245629

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Abstract

Drosophila embryos lacking hindsight gene function have a normal body plan and undergo normal germ-band extension. However, they fail to retract their germ bands. hindsight encodes a large nuclear protein of 1920 amino acids that contains fourteen C2H2-type zinc fingers, and glutamine-rich and proline-rich domains, suggesting that it functions as a transcription factor. Initial embryonic expression of hindsight RNA and protein occurs in the endoderm (midgut) and extraembryonic membrane (amnioserosa) prior to germ-band extension and continues in these tissues beyond the completion of germ-band retraction. Expression also occurs in the developing tracheal system, central and peripheral nervous systems, and the ureter of the Malpighian tubules. Strikingly, hindsight is not expressed in the epidermal ectoderm which is the tissue that undergoes the cell shape changes and movements during germ-band retraction. The embryonic midgut can be eliminated without affecting germ-band retraction. However, elimination of the amnioserosa results in the failure of germ-band retraction, implicating amnioserosal expression of hindsight as crucial for this process. Ubiquitous expression of hindsight in the early embryo rescues germ-band retraction without producing dominant gainof-function defects, suggesting that hindsight’s role in germ-band retraction is permissive rather than instructive. Previous analyses have shown that hindsight is required for maintenance of the differentiated amnioserosa (Frank, L. C. and Rushlow, C. (1996) Development 122, 1343-1352). Two classes of models are consistent with the present data. First, hindsight’s function in germ-band retraction may be limited to maintenance of the amnioserosa which then plays a physical role in the retraction process through contact with cells of the epidermal ectoderm. Second, hindsight might function both to maintain the amnioserosa and to regulate chemical signaling from the amnioserosa to the epidermal ectoderm, thus coordinating the cell shape changes and movements that drive germ-band retraction.


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http://dev.biologists.org/content/124/11/2129PublisherUNSPECIFIED
Additional Information:© 1997 The Company of Biologists Limited. Accepted 25 March 1997. Special thanks to G. Pflugfelder for kindly providing the chromosomal walk in the vicinity of omb that enabled us to clone hnt. Drosophila stocks were provided by the Bloomington Drosophila Stock Center as well as S. Celniker, P. Gergen, A. Hilliker, M. O’Connor, W. Pak, S. Parkhurst, S. Parks, T. Schüpbach and B.-Z. Shilo. Antibodies were kindly provided by S. Beckendorf, S. Benzer, K. Blochlinger, S. Celniker, T. Kaufman, M. Krasnow, Y. Kuo, M. Levine and E. B. Lewis. Thanks to E. Wieschaus for suggesting the hkb tld experiment, S. Ou of the Caltech Monoclonal Antibody Facility for carrying out the hybridoma fusions involved in anti-HNT monoclonal antibody production, K. Zinn and E. B. Lewis for the use of laboratory equipment after the majority of the Lipshitz laboratory moved to Toronto. We thank G. Boulianne, S. Egan, C.c. Hui, R. McInnes and L. Rochwerger for comments on the manuscript. M. L. R. Y. was supported in part by a predoctoral fellowship from the Howard Hughes Medical Institute and M. L. L. by a postdoctoral National Research Service Award from the USPHS (GM16541). This research was supported by research grants to H. D. L. from the American Cancer Society (DB-14) and the National Cancer Institute of Canada (#7447) with funds from the Canadian Cancer Society.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
USPHS National Research Service AwardGM16541
American Cancer SocietyDB-14
National Cancer Institute of Canada7447
Canadian Cancer SocietyUNSPECIFIED
Subject Keywords:hindsight; zinc finger; germ-band retraction; Drosophila; morphogenesis
Record Number:CaltechAUTHORS:20120118-145245629
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120118-145245629
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28841
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Jan 2012 23:23
Last Modified:26 Dec 2012 14:42

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