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Neural tube-ectoderm interactions are required for trigeminal placode formation

Stark, Michael R. and Sechrist, John and Marcelle, Christophe and Bronner, Marianne E. (1997) Neural tube-ectoderm interactions are required for trigeminal placode formation. Development, 124 (21). pp. 4287-4295. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120119-094014765

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Abstract

Cranial sensory ganglia in vertebrates develop from the ectodermal placodes, the neural crest, or both. Although much is known about the neural crest contribution to cranial ganglia, relatively little is known about how placode cells form, invaginate and migrate to their targets. Here, we identify Pax-3 as a molecular marker for placode cells that contribute to the ophthalmic branch of the trigeminal ganglion and use it, in conjunction with DiI labeling of the surface ectoderm, to analyze some of the mechanisms underlying placode development. Pax-3 expression in the ophthalmic placode is observed as early as the 4-somite stage in a narrow band of ectoderm contiguous to the midbrain neural folds. Its expression broadens to a patch of ectoderm adjacent to the midbrain and the rostral hindbrain at the 8- to 10-somite stage. Invagination of the first Pax-3-positive cells begins at the 13-somite stage. Placodal invagination continues through the 35-somite stage, by which time condensation of the trigeminal ganglion has begun. To challenge the normal tissue interactions leading to placode formation, we ablated the cranial neural crest cells or implanted barriers between the neural tube and the ectoderm. Our results demonstrate that, although the presence of neural crest cells is not mandatory for Pax-3 expression in the forming placode, a diffusible signal from the neuroectoderm is required for induction and/or maintenance of the ophthalmic placode.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/124/21/4287PublisherUNSPECIFIED
Additional Information:© 1997 The Company of Biologists Limited. Accepted 11 August 1997. We thank Angela Nieto for early contributions to this work and Martin Goulding for the chick and mouse Pax-3 probe. We are indebted to Brian Rowe, Roham Zamanian, Johnny Choi, and Parisa Zarbafian for technical assistance and Drs Clare Baker, Andrew Groves, Catherine Krull and Ben Murray for critical reading of the manuscript. This work was supported by NS34671 to M. B. F. and a grant from the Muscular Dystrophy Association to C. M.
Funders:
Funding AgencyGrant Number
Public Health ServiceNS34671
Muscular Dystrophy AssociationUNSPECIFIED
Subject Keywords:placode; cranial ganglion; neural crest; Pax-3; FREK; chick
Record Number:CaltechAUTHORS:20120119-094014765
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120119-094014765
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:28850
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Jan 2012 19:17
Last Modified:26 Dec 2012 14:42

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