Yang, Zhong-Nan and Ye, Xin-Rong and Choi, Sandong and Molina, Joe and Moonan, Francis and Wing, Rod A. and Roose, Mikeal L. and Mirkov, T. Erik (2001) Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata (L.) Raf. Genome, 44 (3). pp. 382-393. ISSN 0831-2796 http://resolver.caltech.edu/CaltechAUTHORS:20120119-094409495
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The citrus tristeza virus resistance gene (Ctv) is a single dominant gene in Poncirus trifoliata, a sexually compatible relative of citrus. To clone this gene, a bacterial artificial chromosome (BAC) library has been constructed from an individual plant that was homozygous for Ctv. This library contains 45 696 clones with an average insert size of 80 kb, corresponding to 9.6 genome equivalents. Screening of the BAC library with five chloroplast DNA probes indicated that 0.58% of the BAC clones contained chloroplast-derived inserts. The chromosome walk across the Ctv locus was initiated using three closely linked genetic markers: C19, AD8, and Z16. The walk has been completed and a contig of ca. 1.2 Mb was constructed. Based on new data, the genetic map in the Ctv region was revised, with Ctv being located between AD8-Z16 and C19 at distances of 1.2 and 0.6 cM, respectively. Utilizing DNA fragments isolated from the contig as RFLP markers, the Ctv locus was further mapped to a region of ca. 300 kb. This contig contains several putative disease-resistance genes similar to the rice Xa21 gene, the tomato Cf-2 gene, and the Arabidopsis thaliana RPS2 gene. This library will therefore allow cloning of Ctv and other putative disease-resistance genes.
|Additional Information:||© 2001 NRC Canada. Received October 6, 2000. Accepted February 13, 2001. Published on the NRC Research Press Web site May 16, 2001. The authors thank Juan Luis Campos and Julieta G. Plancarte for their help in DNA purification, Dr. David. A. Frisch for his help in operating the Q-Bot, Drs. Mao Long and Yeisoo Yu for their helpful discussions, and Dr. Ivan L. Ingelbrecht and Ms Caroline M. Herron for their critical review of the manuscript. This work was supported by grants from the California Citrus Research Board (CTV-009); U.S. Department of Agriculture (USDA), Agricultural Research Service grant No. 59-0790-8-51; and USDA Cooperative State Research, Education, and Extension Service (CSREES) grant No. 99-34399-8460.|
|Subject Keywords:||citrus tristeza virus, chromosome walk, resistance gene.|
|Official Citation:||Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata (L.) Raf. Zhong-Nan Yang, Xin-Rong Ye, Sandong Choi, Joe Molina, Francis Moonan, Rod A Wing, Mikeal L Roose, T Erik Mirkov Genome, 2001, 44:(3) 382-393, 10.1139/g01-021|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||19 Jan 2012 18:09|
|Last Modified:||19 Jan 2012 18:12|
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