Lo, Liching and Anderson, David J. (2011) A Cre-Dependent, Anterograde Transsynaptic Viral Tracer for Mapping Output Pathways of Genetically Marked Neurons. Neuron, 72 (6). pp. 938-950. ISSN 0896-6273 http://resolver.caltech.edu/CaltechAUTHORS:20120203-094246153
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Neurotropic viruses that conditionally infect or replicate in molecularly defined neuronal subpopulations, and then spread transsynaptically, are powerful tools for mapping neural pathways. Genetically targetable retrograde transsynaptic tracer viruses are available to map the inputs to specific neuronal subpopulations, but an analogous tool for mapping synaptic outputs is not yet available. Here we describe a Cre recombinase-dependent, anterograde transneuronal tracer, based on the H129 strain of herpes simplex virus (HSV). Application of this virus to transgenic or knockinmice expressing Cre in peripheral neurons of the olfactory epithelium or the retina reveals widespread, polysynaptic labeling of higher-order neurons in the olfactory and visual systems, respectively. Polysynaptic pathways were also labeled from cerebellar Purkinje cells. In each system, the pattern of labeling was consistent with classical circuittracing studies, restricted to neurons, and anterograde specific. These data provide proof-of-principle for a conditional, nondiluting anterograde transsynaptic tracer for mapping synaptic outputs from genetically marked neuronal subpopulations.
|Additional Information:||© 2011 Elsevier Inc. Accepted: December 1, 2011. Published: December 21, 2011. We thank Dr. L. Enquist for the H129 strain of HSV1, advice, and encouragement throughout this project and for feedback on the manuscript, Dr. Jerry Weir for plasmid pGAL10, Dr. Joseph Gogos for OMP-Cre mice, Dr. Markus Meister for help with visual system circuitry, Drs. A. Basbaum and E. Callaway for helpful comments on the manuscript, G. Mosconi and H. Oates-Barker for laboratory management, and G. Mancuso for administrative assistance. This work was supported by NIH grant 1RO1MH070053. D.J.A. is an Investigator of the Howard Hughes Medical Institute.|
|Official Citation:||Liching Lo, David J. Anderson, A Cre-Dependent, Anterograde Transsynaptic Viral Tracer for Mapping Output Pathways of Genetically Marked Neurons, Neuron, Volume 72, Issue 6, 22 December 2011, Pages 938-950, ISSN 0896-6273, 10.1016/j.neuron.2011.12.002. (http://www.sciencedirect.com/science/article/pii/S0896627311010440)|
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|Deposited By:||Ruth Sustaita|
|Deposited On:||03 Feb 2012 17:59|
|Last Modified:||03 Feb 2012 17:59|
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