Cung, Kellye and Slater, Russell L. and Cui, Yue and Jones, Sharon E. and Ahmad, Habib and Naik, Rajesh R. and McAlpine, Michael C. (2011) Rapid, multiplexed microfluidic phage display. Lab on a Chip, 12 (3). pp. 562-565. ISSN 1473-0197 http://resolver.caltech.edu/CaltechAUTHORS:20120203-100512313
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The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets. Surface display techniques allow for efficient handling of large molecular libraries in small volumes. In particular, phage display has emerged as a powerful technology for selecting peptides and proteins with enhanced, target-specific binding affinities. Yet, the process becomes cumbersome and time-consuming when multiple targets are involved.Here we demonstrate for the first time a microfluidic chip capable of identifying high affinity phage displayed peptides for multiple targets in just a single round and without the need for bacterial infection. The chip is shown to be able to yield well-established control consensus sequences while simultaneously identifying new sequences for clinically important targets. Indeed, the confined parameters of the device allow not only for highly controlled assay conditions but also introduce a significant time-reduction to the phage display process. We anticipate that this easily-fabricated, disposable device has the potential to impact areas ranging from fundamental studies of protein, peptide, and molecular interactions, to applications such as fully automated proteomic screening.
|Additional Information:||© 2012 The Royal Society of Chemistry. Received 18th November 2011, Accepted 9th December 2011. First published on the web 09 Dec 2011. We thank Andrew Hsu for valuable discussions and illustrations. This research was supported by the Air Force Office of Scientific Research (AFOSR) (#FA9550-09-1-0096) and by the American Asthma Foundation (#09-0038).|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||03 Feb 2012 21:03|
|Last Modified:||26 Dec 2012 14:47|
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