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Origins of the avian neural crest: the role of neural plate-epidermal interactions

Selleck, Mark A. J. and Bronner, Marianne E. (1995) Origins of the avian neural crest: the role of neural plate-epidermal interactions. Development, 121 (2). pp. 525-538. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120208-110012393

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Abstract

We have investigated the lineage and tissue interactions that result in avian neural crest cell formation from the ectoderm. Presumptive neural plate was grafted adjacent to non-neural ectoderm in whole embryo culture to examine the role of tissue interactions in ontogeny of the neural crest. Our results show that juxtaposition of non-neural ectoderm and presumptive neural plate induces the formation of neural crest cells. Quail/chick recombinations demonstrate that both the prospective neural plate and the prospective epidermis can contribute to the neural crest. When similar neural plate/epidermal confrontations are performed in tissue culture to look at the formation of neural crest derivatives, juxtaposition of epidermis with either early (stages 4–5) or later (stages 6–10) neural plate results in the generation of both melanocytes and sympathoadrenal cells. Interestingly, neural plates isolated from early stages form no neural crest cells, whereas those isolated later give rise to melanocytes but not crest-derived sympathoadrenal cells. Single cell lineage analysis was performed to determine the time at which the neural crest lineage diverges from the epidermal lineage and to elucidate the timing of neural plate/epidermis interactions during normal development. Our results from stage 8 to 10+ embryos show that the neural plate/neural crest lineage segregates from the epidermis around the time of neural tube closure, suggesting that neural induction is still underway at open neural plate stages.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/121/2/525.abstractPublisherUNSPECIFIED
Additional Information:© 1995 The Company of Biologists Limited. Accepted 29 October 1994. We are grateful to Scott Fraser, Mary Dickinson, Talma Scherson, Jack Sechrist and Kristin Artinger for helpful comments on the manuscript. We thank Sheila Kristy and Scott Pauli for their assistance in preparing the manuscript. M. A. J. S. is supported by a Muscular Dystrophy Association research fellowship. This work was supported by US PHS HD-25138 to M. B. F.
Funders:
Funding AgencyGrant Number
Muscular Dystrophy Association UNSPECIFIED
U.S. Public Health ServiceHD-25138
Subject Keywords:cell lineage, chick embryo, ectoderm, induction, neural tube, neurulation, peripheral nervous system, quail embryo
Record Number:CaltechAUTHORS:20120208-110012393
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120208-110012393
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29197
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Feb 2012 23:28
Last Modified:26 Dec 2012 14:48

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