Wang, David G. -W. and Kirchhamer, Carmen V. and Britten, Roy J. and Davidson, Eric H. (1995) SpZ12-1, a negative regulator required for spatial control of the territory-specific CyIIIa gene in the sea urchin embryo. Development, 121 (4). pp. 1111-1122. ISSN 0950-1991 http://resolver.caltech.edu/CaltechAUTHORS:20120216-102559113
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The CyIIIa cytoskeletal actin gene of the sea urchin Strongylocentrotus purpuratus is activated in late cleavage and expressed exclusively in the aboral ectoderm territory of the embryo. Previous gene transfer studies defined a 2.3 kb cis-regulatory region that is necessary and sufficient for correct temporal and spatial expression of a CyIIIa. CAT fusion gene. In this paper, a negative regulatory element within this region was identified that is required for repression of the CyIIIa gene in skeletogenic mesenchyme cells. The repression mediated by this regulatory element takes place after initial territorial specification. A cDNA clone encoding a DNA-binding protein with twelve Zn fingers (SpZ12-1) was isolated by probing an expression library with this cis-element. Deletion analysis of the SpZ12-1 protein confirmed that a DNA-binding domain is located within the Zn finger region. SpZ12-1 is the only DNA-binding protein in embryo nuclear extract that interacts with the specific cis-target sites required for repression of CyIIIa.CAT in skeletogenic mesenchyme and is likely to be the trans factor that mediates this repression.
|Additional Information:||© 1995 Company of Biologists Limited. Accepted 3 January 1995. We thank Dr Christer Höög, Ann E. Cutting and Vikas Duvvuri for technical assistance, Dr Kirsten L. Rood for critically editing a draft of the manuscript, and Professors Ellen Rothenberg and Scott E. Fraser for valuable remarks. This research was supported by NIH Grant HD-05753. D. G. W. was supported by a McCallum Fellowship, California Biochemical Foundation and NIH Grant HD-05753. C. V. K. was supported by a training grant from the Austrian government.|
|Subject Keywords:||transcription factor; sea urchin gene regulation; CyIIIa|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Jason Perez|
|Deposited On:||16 Feb 2012 23:50|
|Last Modified:||26 Dec 2012 14:51|
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