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Violation of cell lineage restriction compartments in the chick hindbrain

Birgbauer, Eric and Fraser, Scott E. (1994) Violation of cell lineage restriction compartments in the chick hindbrain. Development, 120 (6). pp. 1347-1356. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120217-150824375

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Abstract

Previous cell lineage studies indicate that the repeated neuromeres of the chick hindbrain, the rhombomeres, are cell lineage restriction compartments. We have extended these results and tested if the restrictions are absolute. Two different cell marking techniques were used to label cells shortly after rhombomeres form (stage 9+ to 13) so that the resultant clones could be followed up to stage 25. Either small groups of cells were labelled with the lipophilic dye DiI or single cells were injected intracellularly with fluorescent dextran. The majority of the descendants labelled by either technique were restricted to within a single rhombomere. However, in a small but reproducible proportion of the cases (greater than 5%), the clones expanded across a rhombomere boundary. Neither the stage of injection, the stage of analysis, the dorsoventral position, nor the rhombomere identity correlated with the boundary crossing. Judging from the morphology of the cells, both neurons and non-neuronal cells were able to expand over a boundary. These results demonstrate that the rhombomere boundaries represent cell lineage restriction barriers which are not impenetrable in normal development.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/120/6/1347PublisherUNSPECIFIED
Additional Information:© 1994 The Company of Biologists Limited. Accepted 10 March 1994. We would like to thank Amy Bih-Jwo Lin for help with the dextran injections, and Marianne Bronner-Fraser, Jack Sechrist, Bill Trevarrow, and George Serbedzija for critical reading of the manuscript. E. B. is a Fellow of The Jane Coffin Childs Memorial Fund for Medical Research. This investigation has been aided by a grant from The Jane Coffin Childs Memorial Fund for Medical Research and the NIH (HD-26864).
Funders:
Funding AgencyGrant Number
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
NIHHD-26864
Subject Keywords:rhombomere, hindbrain, lineage, compartments, boundaries, LRD, DiI
Record Number:CaltechAUTHORS:20120217-150824375
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120217-150824375
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29372
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Feb 2012 00:45
Last Modified:26 Dec 2012 14:51

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