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Developmental potential of trunk neural crest cells in the mouse

Serbedzija, George N. and Fraser, Scott E. and Bronner, Marianne E. (1994) Developmental potential of trunk neural crest cells in the mouse. Development, 120 (7). pp. 1709-1718. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120221-151101703

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Abstract

The availability of naturally occurring and engineered mutations in mice which affect the neural crest makes the mouse embryo an important experimental system for studying neural crest cell differentiation. Here, we determine the normal developmental potential of neural crest cells by performing in situ cell lineage analysis in the mouse by microinjecting lysinated rhodamine dextran (LRD) into individual dorsal neural tube cells in the trunk. Labeled progeny derived from single cells were found in the neural tube, dorsal root ganglia, sympathoadrenal derivatives, presumptive Schwann cells and/or pigment cells. Most embryos contained labeled cells both in the neural tube and at least one neural crest derivative, and numerous clones contributed to multiple neural crest derivatives. The time of injection influenced the derivatives populated by the labeled cells. Injections at early stages of migration yielded labeled progeny in both dorsal and ventral neural crest derivatives, whereas those performed at later stages had labeled cells only in more dorsal neural crest derivatives, such as dorsal root ganglion and presumptive pigment cells. The results suggest that in the mouse embryo: (1) there is a common precursor for neural crest and neural tube cells; (2) some neural crest cells are multipotent; and (3) the timing of emigration influences the range of possible neural crest derivatives.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/120/7/1709PublisherUNSPECIFIED
Additional Information:© 1994 The Company of Biologists Limited. Accepted 14 April 1994. We thank Andres Collazo for helpful comments on the manuscript and Mary Flowers for excellent technical assistance. This work was supported by USPHS HD-26864 to S. E. F. and HD-25138 to M. B.-F.
Funders:
Funding AgencyGrant Number
U. S. Public Health Service (USPHS)HD-26864
U. S. Public Health Service (USPHS)HD-25138
Subject Keywords:developmental potential, cell fate, neuron, microinjection
Record Number:CaltechAUTHORS:20120221-151101703
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120221-151101703
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29398
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Feb 2012 00:20
Last Modified:26 Dec 2012 14:52

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