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Genetics of the serine cycle in Methylobacterium extorquens AM1: cloning, sequence, mutation, and physiological effect of glyA, the gene for serine hydroxymethyltransferase

Chistoserdova, Ludmila V. and Lidstrom, Mary E. (1994) Genetics of the serine cycle in Methylobacterium extorquens AM1: cloning, sequence, mutation, and physiological effect of glyA, the gene for serine hydroxymethyltransferase. Journal of Bacteriology, 176 (21). pp. 6759-6763. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:20120222-092859484

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Abstract

The gene (glyA) of Methylobacterium extorquens AM1 encoding serine hydroxymethyltransferase (SHMT), one of the key enzymes of the serine cycle for C_1 assimilation, was isolated by using a synthetic oligonucleotide with a sequence based on amino acid sequence conserved in SHMTs from different sources. The amino acid sequence deduced from the gene revealed high similarity to those of known SHMTs. The cloned gene was inactivated by insertion of a kanamycin resistance gene, and recombination of this insertion derivative with the wild-type gene produced an SHMT null mutant. Surprisingly, this mutant had lost its ability to grow on C_1 as well as on C_2 compounds but was still able to grow on succinate. The DNA fragment containing glyA was shown not to be linked with fragments carrying serine cycle genes identified earlier, making it the fourth chromosomal region of M. extorquens AM1 to be indicated as being involved in C_1 assimilation.


Item Type:Article
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http://jb.asm.org/content/176/21/6759PublisherUNSPECIFIED
Additional Information:© 1994 American Society for Microbiology. Received 13 June 1994; Accepted 29 August 1994. This work was supported by a grant from the NIH (GM36294).
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Funding AgencyGrant Number
NIHGM36294
Record Number:CaltechAUTHORS:20120222-092859484
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120222-092859484
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29408
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Feb 2012 00:23
Last Modified:26 Dec 2012 14:52

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