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Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB

Chistoserdova, Ludmila V. and Lidstrom, Mary E. (1994) Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB. Journal of Bacteriology, 176 (23). pp. 7398-7404. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:20120222-094550283

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Abstract

In a recent paper we reported the sequence of the beginning of a serine cycle gene cluster on the Methylobacterium extorquens AM1 chromosome, containing the genes encoding serine glyoxylate aminotransferase (sgaA), hydroxypyruvate reductase (hprA), and 5,10-methylenetetrahydrofolate dehydrogenase (mtdA) (L. V. Chistoserdova and M. E. Lidstrom J. Bacteriol. 176:1957-1968, 1994). Here we present the sequence of the adjacent downstream region containing three full and one partial open reading frames. The first of the full open reading frames (orf4) remains unidentified, while the other two (mtkA and mtkB) code for the two subunits of malate thiokinase, and the fourth, a partial open reading frame (ppcA), apparently encodes phosphoenolpyruvate carboxylase. Mutants containing insertion mutations in orf4, mtdA, and mtdB all were unable to grow on C1 compounds, showing that these three newly identified genes are indispensable for the operation of the serine cycle. Mutants in orf4 were also unable to grow on C_2 compounds, but growth was restored by glyoxylate, suggesting that orf4 might be required for the conversion of acetyl coenzyme A to glyoxylate.


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http://jb.asm.org/content/176/23/7398PublisherUNSPECIFIED
Additional Information:© 1994 American Society for Microbiology. Received 11 July 1994; Accepted 20 September 1994. This work was supported by a grant from the NIH (GM36294).
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NIHGM36294
Record Number:CaltechAUTHORS:20120222-094550283
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120222-094550283
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29410
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Feb 2012 00:22
Last Modified:26 Dec 2012 14:52

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