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Different αl-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins

Wu, Dianqing and Jiang, Huiping and Simon, Melvin I. (1995) Different αl-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins. Journal of Biological Chemistry, 270 (17). pp. 9828-9832. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:20120224-072028827

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Abstract

In order to understand the specific interactions between receptors and guanine nucleotide-binding regulatory protein (G proteins), we attempted to delineate the α1B-adrenergic receptor sequences involved in activation of the α subunits of the Gq class of G proteins. A number of specific mutations were introduced into the third inner loop of the receptor, and the mutants were tested for their abilities to activate different Gα subunits of the Gq class. Our results indicate that the receptor sequences required for activating Gαq/11, Gα14, or Gα16 are different. The sequence extending from residues Lys^(240) to His^(252) is required for activation of Gαq/11, but not for activation of Gα14 or Gα16. Two segments in the third loop of the receptor are required for activation of Gα14: one is located at the N terminus of the loop ending at residue Asn^(226), and the other is located at the C terminus of the loop starting from residue Ser^(278). The latter contains a BB XXB motif, which is apparently critical for Gα14 coupling, but not for Gα16 or Gαq/11 coupling. Furthermore, the three amino acids stretch (Tyr^(217) to Val^(219)) included in the N-terminal segment is not only required for Gα14 coupling, but also for Gαq/11 coupling. It may be involved to some extent in Gα16 coupling as well.


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http://dx.doi.org/10.1074/jbc.270.17.9828DOIUNSPECIFIED
http://www.jbc.org/content/270/17/9828.abstractPublisherUNSPECIFIED
Additional Information:© 1995 by The American Society for Biochemistry and Molecular Biology, Inc. Received for publication, January 19, 1995. This work is supported by a National Institutes of Health grant (to M. I. S.) and a Leukemia Society of America fellowship (to H. J .). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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Record Number:CaltechAUTHORS:20120224-072028827
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120224-072028827
Official Citation:Different α1-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins Dianqing Wu, Huiping Jiang, and Melvin I. Simon J. Biol. Chem. 1995 270: 9828-9832. doi:10.1074/jbc.270.17.9828
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ID Code:29447
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:24 Feb 2012 15:38
Last Modified:24 Feb 2012 15:38

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