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Segmental migration of the hindbrain neural crest does not arise from its segmental generation

Sechrist, J. and Serbedzija, G. N. and Scherson, T. and Fraser, S. E. and Bronner-Fraser, Marianne E. (1993) Segmental migration of the hindbrain neural crest does not arise from its segmental generation. Development, 118 (3). pp. 691-703. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120301-092619387

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Abstract

The proposed pathways of chick cranial neural crest migration and their relationship to the rhombomeres of the hindbrain have been somewhat controversial, with differing results emerging from grafting and DiI-labelling analyses. To resolve this discrepancy, we have examined cranial neural crest migratory pathways using the combination of neurofilament immunocytochemistry, which recognizes early hindbrain neural crest cells, and labelling with the vital dye, DiI. Neurofilament-positive cells with the appearance of premigratory and early-migrating neural crest cells were noted at all axial levels of the hindbrain. At slightly later stages, neural crest cell migration in this region appeared segmented, with no neural crest cells obvious in the mesenchyme lateral to rhombomere 3 (r3) and between the neural tube and the otic vesicle lateral to r5. Focal injections of DiI at the levels of r3 and r5 demonstrated that both of these rhombomeres generated neural crest cells. The segmental distribution of neural crest cells resulted from the DiI-labelled cells that originated in r3 and r5 deviating rostrally or caudally and failing to enter the adjacent preotic mesoderm or otic vesicle region. The observation that neural crest cells originating from r3 and r5 avoided specific neighboring domains raises the intriguing possibility that, as in the trunk, extrinsic factors play a major role in the axial patterning of the cranial neural crest and the neural crest-derived peripheral nervous system.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/content/118/3/691.abstractPublisherUNSPECIFIED
Additional Information:© 1993 The Company of Biologists Limited. Accepted 15 April 1993. We thank Mary Flowers for excellent technical assistance and Dr Mark Selleck and John Wolf for help with some of the DiI injections. We greatly appreciate the critical comments of Drs Andrew Lumsden, Mark Selleck and Claudio Stern on the manuscript. This study was supported by USPHS HD-25138 to M. B.-F. and HD- 29304 to S. E. F.
Funders:
Funding AgencyGrant Number
U. S. Public Health Service (USPHS)HD-25138
U. S. Public Health Service (USPHS)HD-29304
Subject Keywords:neurofilament proteins, DiI labelling, rhombomeres, segmentation, hindbrain
Record Number:CaltechAUTHORS:20120301-092619387
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120301-092619387
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29543
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Mar 2012 22:38
Last Modified:26 Dec 2012 14:54

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