Joseph, Raji E. and Ginder, Nathaniel D. and Hoy, Julie A. and Nix, Jay C. and Fulton, D. Bruce and Honzatko, Richard B. and Andreotti, Amy H. (2012) Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 68 (2). pp. 145-153. ISSN 1744-3091 http://resolver.caltech.edu/CaltechAUTHORS:20120306-092625552
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The crystal structure of the interleukin-2 tyrosine kinase Src homology domain (Itk SH2) is described and it is found that unlike in studies of this domain using NMR spectroscopy, cis-trans-prolyl isomerization is not readily detected in the crystal structure. Based on similarities between the Itk SH2 crystal form and the cis form of the Itk SH2 NMR structure, it is concluded that it is likely that the prolyl imide bond at least in part adopts the cis conformation in the crystal form. However, the lack of high-resolution data and the dynamic nature of the proline-containing loop mean that the precise imide-bond conformation cannot be determined and prolyl cis-trans isomerization in the crystal cannot be ruled out. Given the preponderance of structures that have been solved by X-ray crystallography in the Protein Data Bank, this result supports the notion that prolyl isomerization in folded proteins has been underestimated among known structures. Interestingly, while the precise status of the proline residue is ambiguous, Itk SH2 crystallizes as a domain-swapped dimer. The domain-swapped structure of Itk SH2 is similar to the domain-swapped SH2 domains of Grb2 and Nck, with domain swapping occurring at the β-meander region of all three SH2 domains. Thus, for Itk SH2 structural analysis by NMR spectroscopy and X-ray crystallography revealed very different structural features: proline isomerization versus domain-swapped dimerization, respectively.
|Additional Information:||© 2012 International Union of Crystallography. Received 22 August 2011. Accepted 21 November 2011. Online 25 January 2012. We would like to thank Dr Michael Shogren-Knaak and Abdelhamid M. Azzaz for help with sedimentation-equilibrium experiments. We would also like to thank Dr Yeon-Kyun Shin and Jaeil Shin for help with size-exclusion chromatography. This work was supported by grants from the National Institutes of Health (National Institute of Allergy and Infectious Diseases, AI043957 and AI075150) to AHA and grant NS010546 to RBH. The use of the beamline at the Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the US Department of Energy under Contract No. DE-AC02-05CH11231.|
|Subject Keywords:||IL-2-inducible T-cell kinase; Src homology 2 domain; proline isomerization; domain swapping.|
|Official Citation:||Acta Cryst.(2012).F68,145-153[doi:10.1107/S1744309111049761] Structure of the interleukin-2 tyrosine kinase Src homology 2 domain; comparison between X-ray and NMR-derived structures R. E. Joseph, N. D. Ginder, J. A. Hoy, J. C. Nix, D. B. Fulton, R. B. Honzatko and A. H. Andreotti|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Ruth Sustaita|
|Deposited On:||13 Mar 2012 17:34|
|Last Modified:||26 Dec 2012 14:54|
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