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Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination

Silver, Daniel P. and Spanopoulou, Eugenia and Mulligan, Richard C. and Baltimore, David (1993) Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination. Proceedings of the National Academy of Sciences of the United States of America, 90 (13). pp. 6100-6104. ISSN 0027-8424.

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As a probe of whether RAG-1 and RAG-2 gene products activate other genes or form part of the recombinase itself, certain mutants of the RAG genes were assayed for their ability to activate variable-diversity-joining region [V(D)J] recombination in a plasmid substrate in fibroblasts. The results indicate that the N-terminal one-third of RAG-1, including a zinc-finger-like domain, and an acidic domain of RAG-2 are dispensable for activating V(D)J recombination in a fibroblast, although they contribute quantitatively. In contrast, deletion of the C-terminal segment of RAG-1, which has homology to a topoisomerase-like protein from yeast, abolished recombination activation. These results do not support the hypothesis that the RAG gene products are transcription factors and suggest the possibility that they are parts of the recombination machinery.

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Additional Information:© 1993 National Academy of Sciences. Contributed by David Baltimore, February 22, 1993. We thank Dr. Chris Roman for valuable comments on the manuscript. E.S. was supported by a long-term European Molecular Biology Organization fellowship. This work was supported by National Institutes of Health Grants CA-51462 to D.B. and HL-37569 to R.C.M.
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European Molecular Biology OrganizationUNSPECIFIED
Subject Keywords:recombination activating genes; cysteine-rich motifs; topoisomerase homology
Record Number:CaltechAUTHORS:20120307-154008004
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Official Citation: D P Silver, E Spanopoulou, R C Mulligan, and D Baltimore Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination PNAS 1993 90 (13) 6100-6104
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29631
Deposited By: Ruth Sustaita
Deposited On:12 Mar 2012 20:52
Last Modified:14 Nov 2014 19:21

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