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Dynamic expression of the cell adhesion molecule fasciclin I during embryonic development in Drosophila

McAllister, Linda and Goodman, Corey S. and Zinn, Kai (1992) Dynamic expression of the cell adhesion molecule fasciclin I during embryonic development in Drosophila. Development, 115 (1). pp. 267-276. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20120315-150355858

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Abstract

A number of different cell surface glycoproteins expressed in the central nervous system (CNS) have been identified in insects and shown to mediate cell adhesion in tissue culture systems. The fasciclin I protein is expressed on a subset of CNS axon pathways in both grasshopper and Drosophila. It consists of four homologous 150-amino acid domains which are unrelated to other sequences in the current databases, and is tethered to the cell surface by a glycosyl-phosphatidylinositol linkage. In this paper we examine in detail the expression of fasciclin I mRNA and protein during Drosophila embryonic development. We find that fasciclin I is expressed in several distinct patterns at different stages of development. In blastoderm embryos it is briefly localized in a graded pattern. During the germ band extended period its expression evolves through two distinct phases. Fasciclin I mRNA and protein are initially localized in a 14-stripe pattern which corresponds to segmentally repeated patches of neuroepithelial cells and neuroblasts. Expression then becomes confined to CNS and peripheral sensory (PNS) neurons. Fasciclin I is expressed on all PNS neurons, and this expression is stably maintained for several hours. In the CNS, fasciclin I is initially expressed on all commissural axons, but then becomes restricted to specific axon bundles. The early commissural expression pattern is not observed in grasshopper embryos, but the later bundle-specific pattern is very similar to that seen in grasshopper. The existence of an initial phase of expression on all commissural bundles helps to explain the loss-of-commissures phenotype of embryos lacking expression of both fasciclin I and of the D-abl tyrosine kinase. Fasciclin I is also expressed in several nonneural tissues in the embryo.


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http://dev.biologists.org/content/115/1/267PublisherUNSPECIFIED
Additional Information:© 1992 The Company of Biologists Limited. Accepted 7 February 1992. We thank Shin-Shay Tian for performing some of the whole-mount in situ hybridization experiments, Michael Hortsch for the anti-fascichn I mAbs and for help with initial immunocytochemistry experiments usmg these mAbs, Karen Jepson-Innes and Susan Parkhurst for help with the whole mount in situ technique, Dolores Ferres-Marco for help with immunocytochemistry, Yash Hiromi for a stock carrying the ftz-lacZ insertion on the X chromosome, Bruce Alberts for helpful discussions concerning bicoid phenotypes, and Nipam Patel for invaluable advice. This work was supported by grants from the NIH to C S.G. and K.Z.
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Subject Keywords:adhesion molecule, Drosophila development, axon guidance, neural development
Record Number:CaltechAUTHORS:20120315-150355858
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120315-150355858
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:29736
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Mar 2012 21:41
Last Modified:26 Dec 2012 14:57

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